Extracellular Production, Characterization, and Engineering of a Polyextremotolerant Subtilisin-Like Protease From Feather-Degrading Thermoactinomyces vulgaris Strain CDF

Here, the gene encoding a subtilisin-like protease (protease Als) was cloned from Thermoactinomyces vulgaris strain CDF and expressed in Escherichia coli. The recombinant enzyme was released into the culture medium of E. coli as a mature form (mAls). Purified mAls displayed optimal activity at 60–70...

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Detalles Bibliográficos
Autores principales: Ding, Yidi, Yang, Yong, Ren, Yuxia, Xia, Jingying, Liu, Feng, Li, Yu, Tang, Xiao-Feng, Tang, Bing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779483/
https://www.ncbi.nlm.nih.gov/pubmed/33408708
http://dx.doi.org/10.3389/fmicb.2020.605771

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779483/
https://www.ncbi.nlm.nih.gov/pubmed/33408708
http://dx.doi.org/10.3389/fmicb.2020.605771

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