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Characterization of the adiponectin promoter + Cre recombinase insertion in the Tg(Adipoq-cre)1Evdr mouse by targeted locus amplification and droplet digital PCR

The Tg(Adipoq-cre)1Evdr mouse has become an important tool in adipose tissue biology. However, the exact genomic transgene integration site has not been established. Using Targeted Locus Amplification (TLA) we found the transgene had integrated on mouse chromosome 9 between exons 6 and 7 of Tbx18. W...

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Detalles Bibliográficos
Autores principales: Wong, Adrian M., Patel, Tushar P., Altman, Elizabeth K., Tugarinov, Nicol, Trivellin, Giampaolo, Yanovski, Jack A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781622/
https://www.ncbi.nlm.nih.gov/pubmed/33371773
http://dx.doi.org/10.1080/21623945.2020.1861728