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Trigeminal neuropathic pain is alleviated by inhibition of Ca(v)3.3 T-type calcium channels in mice
In this brief report, we demonstrate that the Ca(v)3.3 T-type voltage-gated calcium channel subtype is involved in our FRICT-ION model of chronic trigeminal neuropathic pain. We first showed that the Cacna1i gene encoding Ca(v)3.3 is significantly upregulated in whole trigeminal ganglia of FRICT-ION...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781641/ https://www.ncbi.nlm.nih.gov/pubmed/33283622 http://dx.doi.org/10.1080/19336950.2020.1859248 |
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author | Montera, Marena Goins, Aleyah Cmarko, Leos Weiss, Norbert Westlund, Karin N. Alles, Sascha R. A. |
author_facet | Montera, Marena Goins, Aleyah Cmarko, Leos Weiss, Norbert Westlund, Karin N. Alles, Sascha R. A. |
author_sort | Montera, Marena |
collection | PubMed |
description | In this brief report, we demonstrate that the Ca(v)3.3 T-type voltage-gated calcium channel subtype is involved in our FRICT-ION model of chronic trigeminal neuropathic pain. We first showed that the Cacna1i gene encoding Ca(v)3.3 is significantly upregulated in whole trigeminal ganglia of FRICT-ION mice compared to controls at week 10 post-injury. We confirmed protein upregulation of Ca(v)3.3 compared to controls using Western blot analysis of whole trigeminal ganglia tissues. Finally, we demonstrated that intraperitoneal injection of a selective TAT-based Ca(v)3.3 blocking peptide in FRICT-ION mice significantly reduces Ca(v)3.3 protein expression at the peak anti-allodynic effect (4 hrs post-injection) of the attenuated neuropathic pain behavior. We also suggest that blockade of Ca(v)3.3 may be more effective in attenuating trigeminal neuropathic pain in female than male FRICT-ION mice. Therefore, blocking or attenuating Ca(v)3.3 function may be an effective strategy for the treatment of trigeminal neuropathic pain. |
format | Online Article Text |
id | pubmed-7781641 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-77816412021-01-14 Trigeminal neuropathic pain is alleviated by inhibition of Ca(v)3.3 T-type calcium channels in mice Montera, Marena Goins, Aleyah Cmarko, Leos Weiss, Norbert Westlund, Karin N. Alles, Sascha R. A. Channels (Austin) Brief Report In this brief report, we demonstrate that the Ca(v)3.3 T-type voltage-gated calcium channel subtype is involved in our FRICT-ION model of chronic trigeminal neuropathic pain. We first showed that the Cacna1i gene encoding Ca(v)3.3 is significantly upregulated in whole trigeminal ganglia of FRICT-ION mice compared to controls at week 10 post-injury. We confirmed protein upregulation of Ca(v)3.3 compared to controls using Western blot analysis of whole trigeminal ganglia tissues. Finally, we demonstrated that intraperitoneal injection of a selective TAT-based Ca(v)3.3 blocking peptide in FRICT-ION mice significantly reduces Ca(v)3.3 protein expression at the peak anti-allodynic effect (4 hrs post-injection) of the attenuated neuropathic pain behavior. We also suggest that blockade of Ca(v)3.3 may be more effective in attenuating trigeminal neuropathic pain in female than male FRICT-ION mice. Therefore, blocking or attenuating Ca(v)3.3 function may be an effective strategy for the treatment of trigeminal neuropathic pain. Taylor & Francis 2020-12-23 /pmc/articles/PMC7781641/ /pubmed/33283622 http://dx.doi.org/10.1080/19336950.2020.1859248 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Brief Report Montera, Marena Goins, Aleyah Cmarko, Leos Weiss, Norbert Westlund, Karin N. Alles, Sascha R. A. Trigeminal neuropathic pain is alleviated by inhibition of Ca(v)3.3 T-type calcium channels in mice |
title | Trigeminal neuropathic pain is alleviated by inhibition of Ca(v)3.3 T-type calcium channels in mice |
title_full | Trigeminal neuropathic pain is alleviated by inhibition of Ca(v)3.3 T-type calcium channels in mice |
title_fullStr | Trigeminal neuropathic pain is alleviated by inhibition of Ca(v)3.3 T-type calcium channels in mice |
title_full_unstemmed | Trigeminal neuropathic pain is alleviated by inhibition of Ca(v)3.3 T-type calcium channels in mice |
title_short | Trigeminal neuropathic pain is alleviated by inhibition of Ca(v)3.3 T-type calcium channels in mice |
title_sort | trigeminal neuropathic pain is alleviated by inhibition of ca(v)3.3 t-type calcium channels in mice |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781641/ https://www.ncbi.nlm.nih.gov/pubmed/33283622 http://dx.doi.org/10.1080/19336950.2020.1859248 |
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