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Maf1 Ameliorates Sepsis-Associated Encephalopathy by Suppressing the NF-kB/NLRP3 Inflammasome Signaling Pathway

BACKGROUND: The NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome has been identified as an important mediator of blood–brain-barrier disruption in sepsis-associated encephalopathy (SAE). However, no information is available concerning the critical upstream regulators of SAE. MET...

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Autores principales: Chen, Shenglong, Tang, Chaogang, Ding, Hongguang, Wang, Zhonghua, Liu, Xinqiang, Chai, Yunfei, Jiang, Wenqiang, Han, Yongli, Zeng, Hongke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7785707/
https://www.ncbi.nlm.nih.gov/pubmed/33424842
http://dx.doi.org/10.3389/fimmu.2020.594071
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author Chen, Shenglong
Tang, Chaogang
Ding, Hongguang
Wang, Zhonghua
Liu, Xinqiang
Chai, Yunfei
Jiang, Wenqiang
Han, Yongli
Zeng, Hongke
author_facet Chen, Shenglong
Tang, Chaogang
Ding, Hongguang
Wang, Zhonghua
Liu, Xinqiang
Chai, Yunfei
Jiang, Wenqiang
Han, Yongli
Zeng, Hongke
author_sort Chen, Shenglong
collection PubMed
description BACKGROUND: The NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome has been identified as an important mediator of blood–brain-barrier disruption in sepsis-associated encephalopathy (SAE). However, no information is available concerning the critical upstream regulators of SAE. METHODS: Lipopolysaccharide (LPS) was used to establish an in vitro model of blood–brain barrier (BBB) disruption and an in vivo model of SAE. Disruption of BBB integrity was assessed by measuring the expression levels of tight-junction proteins. NLRP3 inflammasome activation, pro-inflammatory cytokines levels, and neuroapoptosis were measured using biochemical assays. Finally, the FITC-dextran Transwell assay and Evan’s blue dye assay were used to assess the effect of Maf1 on LPS-induced endothelial permeability in vitro and in vivo. RESULTS: We found that Maf1 significantly suppressed the brain inflammatory response and neuroapoptosis induced by LPS in vivo and in vitro. Notably, Maf1 downregulated activation of the NF-κB/p65-induced NLRP3 inflammasome and the expression of pro-inflammatory cytokines. In addition, we found that Maf1 and p65 directly bound to the NLRP3 gene promoter region and competitively regulated the function of NLRP3 in inflammations. Moreover, overexpression of NLRP3 reversed the effects of p65 on BBB integrity, apoptosis, and inflammation in response to LPS. Our study revealed novel role for Maf1 in regulating NF-κB-mediated inflammasome formation, which plays a prominent role in SAE. CONCLUSIONS: Regulation of Maf1 might be a therapeutic strategy for SAE and other neurodegenerative diseases associated with inflammation.
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spelling pubmed-77857072021-01-07 Maf1 Ameliorates Sepsis-Associated Encephalopathy by Suppressing the NF-kB/NLRP3 Inflammasome Signaling Pathway Chen, Shenglong Tang, Chaogang Ding, Hongguang Wang, Zhonghua Liu, Xinqiang Chai, Yunfei Jiang, Wenqiang Han, Yongli Zeng, Hongke Front Immunol Immunology BACKGROUND: The NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome has been identified as an important mediator of blood–brain-barrier disruption in sepsis-associated encephalopathy (SAE). However, no information is available concerning the critical upstream regulators of SAE. METHODS: Lipopolysaccharide (LPS) was used to establish an in vitro model of blood–brain barrier (BBB) disruption and an in vivo model of SAE. Disruption of BBB integrity was assessed by measuring the expression levels of tight-junction proteins. NLRP3 inflammasome activation, pro-inflammatory cytokines levels, and neuroapoptosis were measured using biochemical assays. Finally, the FITC-dextran Transwell assay and Evan’s blue dye assay were used to assess the effect of Maf1 on LPS-induced endothelial permeability in vitro and in vivo. RESULTS: We found that Maf1 significantly suppressed the brain inflammatory response and neuroapoptosis induced by LPS in vivo and in vitro. Notably, Maf1 downregulated activation of the NF-κB/p65-induced NLRP3 inflammasome and the expression of pro-inflammatory cytokines. In addition, we found that Maf1 and p65 directly bound to the NLRP3 gene promoter region and competitively regulated the function of NLRP3 in inflammations. Moreover, overexpression of NLRP3 reversed the effects of p65 on BBB integrity, apoptosis, and inflammation in response to LPS. Our study revealed novel role for Maf1 in regulating NF-κB-mediated inflammasome formation, which plays a prominent role in SAE. CONCLUSIONS: Regulation of Maf1 might be a therapeutic strategy for SAE and other neurodegenerative diseases associated with inflammation. Frontiers Media S.A. 2020-12-23 /pmc/articles/PMC7785707/ /pubmed/33424842 http://dx.doi.org/10.3389/fimmu.2020.594071 Text en Copyright © 2020 Chen, Tang, Ding, Wang, Liu, Chai, Jiang, Han and Zeng http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Chen, Shenglong
Tang, Chaogang
Ding, Hongguang
Wang, Zhonghua
Liu, Xinqiang
Chai, Yunfei
Jiang, Wenqiang
Han, Yongli
Zeng, Hongke
Maf1 Ameliorates Sepsis-Associated Encephalopathy by Suppressing the NF-kB/NLRP3 Inflammasome Signaling Pathway
title Maf1 Ameliorates Sepsis-Associated Encephalopathy by Suppressing the NF-kB/NLRP3 Inflammasome Signaling Pathway
title_full Maf1 Ameliorates Sepsis-Associated Encephalopathy by Suppressing the NF-kB/NLRP3 Inflammasome Signaling Pathway
title_fullStr Maf1 Ameliorates Sepsis-Associated Encephalopathy by Suppressing the NF-kB/NLRP3 Inflammasome Signaling Pathway
title_full_unstemmed Maf1 Ameliorates Sepsis-Associated Encephalopathy by Suppressing the NF-kB/NLRP3 Inflammasome Signaling Pathway
title_short Maf1 Ameliorates Sepsis-Associated Encephalopathy by Suppressing the NF-kB/NLRP3 Inflammasome Signaling Pathway
title_sort maf1 ameliorates sepsis-associated encephalopathy by suppressing the nf-kb/nlrp3 inflammasome signaling pathway
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7785707/
https://www.ncbi.nlm.nih.gov/pubmed/33424842
http://dx.doi.org/10.3389/fimmu.2020.594071
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