Cargando…

Simultaneous Determination of Ropivacaine and 3-Hydroxy Ropivacaine in Cerebrospinal Fluid by UPLC-MS/MS

In this paper, a UPLC-MS/MS method was developed for the determination of ropivacaine and its metabolite 3-hydroxy ropivacaine in cerebrospinal fluid. The cerebrospinal fluid was processed by ethyl acetate liquid-liquid extraction. The multiple reaction monitoring (MRM) mode was used for quantitativ...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Siyuan, Ma, Jianshe, Wang, Xianqin, Zhou, Quan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787762/
https://www.ncbi.nlm.nih.gov/pubmed/33490261
http://dx.doi.org/10.1155/2020/8844866
_version_ 1783632890843627520
author Chen, Siyuan
Ma, Jianshe
Wang, Xianqin
Zhou, Quan
author_facet Chen, Siyuan
Ma, Jianshe
Wang, Xianqin
Zhou, Quan
author_sort Chen, Siyuan
collection PubMed
description In this paper, a UPLC-MS/MS method was developed for the determination of ropivacaine and its metabolite 3-hydroxy ropivacaine in cerebrospinal fluid. The cerebrospinal fluid was processed by ethyl acetate liquid-liquid extraction. The multiple reaction monitoring (MRM) mode was used for quantitative analysis by monitoring the transitions of m/z 275.3 → 126.2 for ropivacaine, m/z 291.0 → 126.0 for 3-hydroxy ropivacaine, and m/z 290.2 → 198.2 for the internal standard. Standard curves for ropivacaine and 3-hydroxy ropivacaine in cerebrospinal fluid were conducted over the concentration range of 0.2–2000 ng/mL, demonstrating excellent linearity, and the lower limit of quantification was 0.2 ng/mL. The intraday precision of ropivacaine and 3-hydroxy ropivacaine was less than 11%, while the interday precision was less than 7%. The accuracy ranged between 87% and 107%, the average extraction efficiency was higher than 79%, and the matrix effect was between 89% and 98%. The developed method was then applied to a case of suspected poisoning of ropivacaine.
format Online
Article
Text
id pubmed-7787762
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-77877622021-01-22 Simultaneous Determination of Ropivacaine and 3-Hydroxy Ropivacaine in Cerebrospinal Fluid by UPLC-MS/MS Chen, Siyuan Ma, Jianshe Wang, Xianqin Zhou, Quan Biomed Res Int Research Article In this paper, a UPLC-MS/MS method was developed for the determination of ropivacaine and its metabolite 3-hydroxy ropivacaine in cerebrospinal fluid. The cerebrospinal fluid was processed by ethyl acetate liquid-liquid extraction. The multiple reaction monitoring (MRM) mode was used for quantitative analysis by monitoring the transitions of m/z 275.3 → 126.2 for ropivacaine, m/z 291.0 → 126.0 for 3-hydroxy ropivacaine, and m/z 290.2 → 198.2 for the internal standard. Standard curves for ropivacaine and 3-hydroxy ropivacaine in cerebrospinal fluid were conducted over the concentration range of 0.2–2000 ng/mL, demonstrating excellent linearity, and the lower limit of quantification was 0.2 ng/mL. The intraday precision of ropivacaine and 3-hydroxy ropivacaine was less than 11%, while the interday precision was less than 7%. The accuracy ranged between 87% and 107%, the average extraction efficiency was higher than 79%, and the matrix effect was between 89% and 98%. The developed method was then applied to a case of suspected poisoning of ropivacaine. Hindawi 2020-12-04 /pmc/articles/PMC7787762/ /pubmed/33490261 http://dx.doi.org/10.1155/2020/8844866 Text en Copyright © 2020 Siyuan Chen et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chen, Siyuan
Ma, Jianshe
Wang, Xianqin
Zhou, Quan
Simultaneous Determination of Ropivacaine and 3-Hydroxy Ropivacaine in Cerebrospinal Fluid by UPLC-MS/MS
title Simultaneous Determination of Ropivacaine and 3-Hydroxy Ropivacaine in Cerebrospinal Fluid by UPLC-MS/MS
title_full Simultaneous Determination of Ropivacaine and 3-Hydroxy Ropivacaine in Cerebrospinal Fluid by UPLC-MS/MS
title_fullStr Simultaneous Determination of Ropivacaine and 3-Hydroxy Ropivacaine in Cerebrospinal Fluid by UPLC-MS/MS
title_full_unstemmed Simultaneous Determination of Ropivacaine and 3-Hydroxy Ropivacaine in Cerebrospinal Fluid by UPLC-MS/MS
title_short Simultaneous Determination of Ropivacaine and 3-Hydroxy Ropivacaine in Cerebrospinal Fluid by UPLC-MS/MS
title_sort simultaneous determination of ropivacaine and 3-hydroxy ropivacaine in cerebrospinal fluid by uplc-ms/ms
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787762/
https://www.ncbi.nlm.nih.gov/pubmed/33490261
http://dx.doi.org/10.1155/2020/8844866
work_keys_str_mv AT chensiyuan simultaneousdeterminationofropivacaineand3hydroxyropivacaineincerebrospinalfluidbyuplcmsms
AT majianshe simultaneousdeterminationofropivacaineand3hydroxyropivacaineincerebrospinalfluidbyuplcmsms
AT wangxianqin simultaneousdeterminationofropivacaineand3hydroxyropivacaineincerebrospinalfluidbyuplcmsms
AT zhouquan simultaneousdeterminationofropivacaineand3hydroxyropivacaineincerebrospinalfluidbyuplcmsms