Joint neutron/X-ray crystal structure of a mechanistically relevant complex of perdeuterated urate oxidase and simulations provide insight into the hydration step of catalysis

Cofactor-independent urate oxidase (UOX) is an ∼137 kDa tetrameric enzyme essential for uric acid (UA) catabolism in many organisms. UA is first oxidized by O(2) to de­hydro­isourate (DHU) via a peroxo intermediate. DHU then undergoes hydration to 5-hy­droxy­isourate (5HIU). At different stages of t...

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Detalles Bibliográficos
Autores principales: McGregor, Lindsay, Földes, Tamás, Bui, Soi, Moulin, Martine, Coquelle, Nicolas, Blakeley, Matthew P., Rosta, Edina, Steiner, Roberto A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2021
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7792999/
https://www.ncbi.nlm.nih.gov/pubmed/33520242
http://dx.doi.org/10.1107/S2052252520013615

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7792999/
https://www.ncbi.nlm.nih.gov/pubmed/33520242
http://dx.doi.org/10.1107/S2052252520013615

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