Cargando…

Joint neutron/X-ray crystal structure of a mechanistically relevant complex of perdeuterated urate oxidase and simulations provide insight into the hydration step of catalysis

Cofactor-independent urate oxidase (UOX) is an ∼137 kDa tetrameric enzyme essential for uric acid (UA) catabolism in many organisms. UA is first oxidized by O(2) to dehydroisourate (DHU) via a peroxo intermediate. DHU then undergoes hydration to 5-hydroxyisourate (5HIU). At different stages of t...

Descripción completa

Detalles Bibliográficos
Autores principales:	McGregor, Lindsay, Földes, Tamás, Bui, Soi, Moulin, Martine, Coquelle, Nicolas, Blakeley, Matthew P., Rosta, Edina, Steiner, Roberto A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	International Union of Crystallography 2021
Materias:	Research Papers
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7792999/ https://www.ncbi.nlm.nih.gov/pubmed/33520242 http://dx.doi.org/10.1107/S2052252520013615

Ejemplares similares

Microgravity crystallization of perdeuterated tryptophan synthase for neutron diffraction
por: Drago, Victoria N., et al.
Publicado: (2022)

Perdeuterated GbpA Enables Neutron Scattering Experiments of a Lytic Polysaccharide Monooxygenase
por: Sørensen, H. V., et al.
Publicado: (2023)

The Neutron Structure of Urate Oxidase Resolves a Long-Standing Mechanistic Conundrum and Reveals Unexpected Changes in Protonation
por: Oksanen, Esko, et al.
Publicado: (2014)

Production of perdeuterated fucose from glyco-engineered bacteria
por: Gajdos, Lukas, et al.
Publicado: (2020)

Perdeuteration, crystallization, data collection and comparison of five neutron diffraction data sets of complexes of human galectin-3C
por: Manzoni, Francesco, et al.
Publicado: (2016)

Binding site asymmetry in human transthyretin: insights from a joint neutron and X-ray crystallographic analysis using perdeuterated protein
por: Haupt, Melina, et al.
Publicado: (2014)

Direct Evidence for a Peroxide Intermediate and a Reactive Enzyme–Substrate–Dioxygen Configuration in a Cofactor-free Oxidase**
por: Bui, Soi, et al.
Publicado: (2014)

Catalysis and Structure of Zebrafish Urate Oxidase Provide Insights into the Origin of Hyperuricemia in Hominoids
por: Marchetti, Marialaura, et al.
Publicado: (2016)

Structural analysis of urate oxidase in complex with its natural substrate inhibited by cyanide: Mechanistic implications
por: Gabison, Laure, et al.
Publicado: (2008)

Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells
por: de Ghellinck, Alexis, et al.
Publicado: (2014)

Perdeuterated Conjugated Polymers for Ultralow‐Frequency Magnetic Resonance of OLEDs
por: Milster, Sebastian, et al.
Publicado: (2020)

An Optical Urate Biosensor Based on Urate Oxidase and Long-Lifetime Metalloporphyrins
por: Falohun, Tokunbo, et al.
Publicado: (2020)

Calcium Inhibition of Ribonuclease H1 Two-Metal Ion Catalysis
por: Rosta, Edina, et al.
Publicado: (2014)

Metalloprotein catalysis: structural and mechanistic insights into oxidoreductases from neutron protein crystallography
por: Schröder, Gabriela C., et al.
Publicado: (2021)

Toward Understanding CB[7]-Based Supramolecular Diels-Alder Catalysis
por: Berta, Dénes, et al.
Publicado: (2020)

Evolutionary adaptation from hydrolytic to oxygenolytic catalysis at the α/β-hydrolase fold
por: Bui, Soi, et al.
Publicado: (2023)

Turning Cucurbit[8]uril into a Supramolecular Nanoreactor for Asymmetric Catalysis
por: Zheng, Lifei, et al.
Publicado: (2015)

Turning Cucurbit[8]uril into a Supramolecular Nanoreactor for Asymmetric Catalysis
por: Zheng, Lifei, et al.
Publicado: (2015)

Urate Oxidase Purification by Salting-in Crystallization: Towards an Alternative to Chromatography
por: Giffard, Marion, et al.
Publicado: (2011)

Cloning and expression of Aspergillus flavus urate oxidase in Pichia pastoris
por: Fazel, Ramin, et al.
Publicado: (2014)

Collective Mid-Infrared Vibrations in Surface-Enhanced Raman Scattering
por: Mueller, Niclas S., et al.
Publicado: (2022)

Structural insights into protein folding, stability and activity using in vivo perdeuteration of hen egg-white lysozyme
por: Ramos, Joao, et al.
Publicado: (2021)

Neutron structures of Leishmania mexicana triosephosphate isomerase in complex with reaction-intermediate mimics shed light on the proton-shuttling steps
por: Kelpšas, Vinardas, et al.
Publicado: (2021)

Role of urate, xanthine oxidase and the effects of allopurinol in vascular oxidative stress
por: George, Jacob, et al.
Publicado: (2009)

The role of urate and xanthine oxidase in vascular oxidative stress: future directions
por: George, Jacob, et al.
Publicado: (2009)

Thermostable and Long-Circulating Albumin-Conjugated Arthrobacter globiformis Urate Oxidase
por: Yang, Byungseop, et al.
Publicado: (2021)

A high-performance electrochemical biosensor using an engineered urate oxidase
por: Wei, Zheng, et al.
Publicado: (2023)

Back-exchange of deuterium in neutron crystallography: characterization by IR spectroscopy
por: Yee, Ai Woon, et al.
Publicado: (2017)

Enhancing protein perdeuteration by experimental evolution of Escherichia coli K‐12 for rapid growth in deuterium‐based media
por: Kelpšas, Vinardas, et al.
Publicado: (2021)

Neutron Scattering Studies of Heterogeneous Catalysis
por: Yu, Xinbin, et al.
Publicado: (2023)

An experimental point of view on hydration/solvation in halophilic proteins
por: Talon, Romain, et al.
Publicado: (2014)

Directed evolution to improve the catalytic efficiency of urate oxidase from Bacillus subtilis
por: Li, Wenjie, et al.
Publicado: (2017)

Strong structuring arising from weak cooperative O-H···π and C-H···O hydrogen bonding in benzene-methanol solution
por: Di Mino, Camilla, et al.
Publicado: (2023)

Neutron crystallography aids in drug design
por: Blakeley, M. P.
Publicado: (2016)

REDCRAFT: A computational platform using residual dipolar coupling NMR data for determining structures of perdeuterated proteins in solution
por: Cole, Casey A., et al.
Publicado: (2021)

In-Situ Spectro-Electrochemistry of Conductive Polymers Using Plasmonics to Reveal Doping Mechanisms
por: Peng, Jialong, et al.
Publicado: (2022)

Natural heterogeneous catalysis with immobilised oxidase biocatalysts
por: Mattey, Ashley P., et al.
Publicado: (2020)

An In Vitro Catalysis of Tea Polyphenols by Polyphenol Oxidase
por: Liu, Kunyi, et al.
Publicado: (2023)

Urate oxidase knockdown decreases oxidative stress in a murine hepatic cell line
por: Cleveland, Beth M, et al.
Publicado: (2009)

Cloning, Purification, and Partial Characterization of Bacillus subtilis Urate Oxidase Expressed in Escherichia coli
por: Pfrimer, Pollyana, et al.
Publicado: (2010)

Cannot write session to /tmp/vufind_sessions/sess_o3kghboq7cfupoc3nrodfu7dsb