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Switching between Ultrafast Pathways Enables a Green-Red Emission Ratiometric Fluorescent-Protein-Based Ca(2+) Biosensor

Ratiometric indicators with long emission wavelengths are highly preferred in modern bioimaging and life sciences. Herein, we elucidated the working mechanism of a standalone red fluorescent protein (FP)-based Ca(2+) biosensor, REX-GECO1, using a series of spectroscopic and computational methods. Up...

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Detalles Bibliográficos
Autores principales: Tang, Longteng, Zhang, Shuce, Zhao, Yufeng, Rozanov, Nikita D., Zhu, Liangdong, Wu, Jiahui, Campbell, Robert E., Fang, Chong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794744/
https://www.ncbi.nlm.nih.gov/pubmed/33466257
http://dx.doi.org/10.3390/ijms22010445