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Bio-Absorption of Human Dentin-Derived Biomaterial in Sheep Critical-Size Iliac Defects
The aim of this study was to evaluate the bio-absorption and bone regeneration of human tooth-derived dentin scaffold, entitled as perforated root-demineralized dentin matrix (PR-DDM), after in vivo implantation into the critical-size iliac defects. The dentin scaffolds were prepared from human vita...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796471/ https://www.ncbi.nlm.nih.gov/pubmed/33466422 http://dx.doi.org/10.3390/ma14010223 |
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author | Kabir, Md Arafat Murata, Masaru Shakya, Mamata Yamada, Katsuhisa Akazawa, Toshiyuki |
author_facet | Kabir, Md Arafat Murata, Masaru Shakya, Mamata Yamada, Katsuhisa Akazawa, Toshiyuki |
author_sort | Kabir, Md Arafat |
collection | PubMed |
description | The aim of this study was to evaluate the bio-absorption and bone regeneration of human tooth-derived dentin scaffold, entitled as perforated root-demineralized dentin matrix (PR-DDM), after in vivo implantation into the critical-size iliac defects. The dentin scaffolds were prepared from human vital, non-functional teeth. Thirty artificial macro-pores (Ø 1 mm) were added after removing the enamel portion. The modified teeth were supersonically demineralized in 0.34 N HNO(3) for 30 min. The microstructure was observed by scanning electron microscope (SEM). The 3D micro-CT and histological analysis were carried out to evaluate the bio-absorption of PR-DDM at 2 and 4 months. A smooth dentin collagen surface with symmetrical macro-pores and tube-type dentinal tubules (Ø 1–2 µm) with micro-cracks were observed on the perforated region. A significant number of custom-made macro-pores disappeared, and the size of the macro-pores became significantly wider at 4 months compared with the 2 months (p < 0.05) evaluated by 3D micro-CT. Histological images revealed the presence of multinucleated giant cells attached to the scalloped border of the PR-DDM. The morphological changes due to bio-absorption by the cellular phagocytes were comparable to the 3D micro-CT and histological images at 2 and 4 months. Altogether, the results demonstrated that the PR-DDM block was gradually absorbed by multinucleated giant cells and regenerated bone. Human PR-DDM might serve as a unique scaffold for extraoral bone regeneration. |
format | Online Article Text |
id | pubmed-7796471 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-77964712021-01-10 Bio-Absorption of Human Dentin-Derived Biomaterial in Sheep Critical-Size Iliac Defects Kabir, Md Arafat Murata, Masaru Shakya, Mamata Yamada, Katsuhisa Akazawa, Toshiyuki Materials (Basel) Article The aim of this study was to evaluate the bio-absorption and bone regeneration of human tooth-derived dentin scaffold, entitled as perforated root-demineralized dentin matrix (PR-DDM), after in vivo implantation into the critical-size iliac defects. The dentin scaffolds were prepared from human vital, non-functional teeth. Thirty artificial macro-pores (Ø 1 mm) were added after removing the enamel portion. The modified teeth were supersonically demineralized in 0.34 N HNO(3) for 30 min. The microstructure was observed by scanning electron microscope (SEM). The 3D micro-CT and histological analysis were carried out to evaluate the bio-absorption of PR-DDM at 2 and 4 months. A smooth dentin collagen surface with symmetrical macro-pores and tube-type dentinal tubules (Ø 1–2 µm) with micro-cracks were observed on the perforated region. A significant number of custom-made macro-pores disappeared, and the size of the macro-pores became significantly wider at 4 months compared with the 2 months (p < 0.05) evaluated by 3D micro-CT. Histological images revealed the presence of multinucleated giant cells attached to the scalloped border of the PR-DDM. The morphological changes due to bio-absorption by the cellular phagocytes were comparable to the 3D micro-CT and histological images at 2 and 4 months. Altogether, the results demonstrated that the PR-DDM block was gradually absorbed by multinucleated giant cells and regenerated bone. Human PR-DDM might serve as a unique scaffold for extraoral bone regeneration. MDPI 2021-01-05 /pmc/articles/PMC7796471/ /pubmed/33466422 http://dx.doi.org/10.3390/ma14010223 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kabir, Md Arafat Murata, Masaru Shakya, Mamata Yamada, Katsuhisa Akazawa, Toshiyuki Bio-Absorption of Human Dentin-Derived Biomaterial in Sheep Critical-Size Iliac Defects |
title | Bio-Absorption of Human Dentin-Derived Biomaterial in Sheep Critical-Size Iliac Defects |
title_full | Bio-Absorption of Human Dentin-Derived Biomaterial in Sheep Critical-Size Iliac Defects |
title_fullStr | Bio-Absorption of Human Dentin-Derived Biomaterial in Sheep Critical-Size Iliac Defects |
title_full_unstemmed | Bio-Absorption of Human Dentin-Derived Biomaterial in Sheep Critical-Size Iliac Defects |
title_short | Bio-Absorption of Human Dentin-Derived Biomaterial in Sheep Critical-Size Iliac Defects |
title_sort | bio-absorption of human dentin-derived biomaterial in sheep critical-size iliac defects |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796471/ https://www.ncbi.nlm.nih.gov/pubmed/33466422 http://dx.doi.org/10.3390/ma14010223 |
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