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Evaluating cytokine production by flow cytometry using brefeldin A in mice

Characterizing cytokine production in situ is important for properly understanding immunologic responses. Cytokine reporter mice are limited by the need to cross markers into various knockout backgrounds and by availability of reporters of interest. To overcome this, we utilize injection of brefeldi...

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Detalles Bibliográficos
Autores principales: Kovacs, Stephen B., Oh, Changhoon, Aachoui, Youssef, Miao, Edward A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797915/
https://www.ncbi.nlm.nih.gov/pubmed/33458706
http://dx.doi.org/10.1016/j.xpro.2020.100244