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Preparation and culture of bone marrow-derived macrophages from mice for functional analysis

The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This pro...

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Detalles Bibliográficos
Autores principales: Toda, Gotaro, Yamauchi, Toshimasa, Kadowaki, Takashi, Ueki, Kohjiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797923/
https://www.ncbi.nlm.nih.gov/pubmed/33458708
http://dx.doi.org/10.1016/j.xpro.2020.100246