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The role of microglia membrane potential in chemotaxis

Microglia react to danger signals by rapid and targeted extension of cellular processes towards the source of the signal. This positive chemotactic response is accompanied by a hyperpolarization of the microglia membrane. Here, we show that optogenetic depolarization of microglia has little effect o...

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Autores principales: Laprell, Laura, Schulze, Christian, Brehme, Marie-Luise, Oertner, Thomas G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798195/
https://www.ncbi.nlm.nih.gov/pubmed/33423699
http://dx.doi.org/10.1186/s12974-020-02048-0
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author Laprell, Laura
Schulze, Christian
Brehme, Marie-Luise
Oertner, Thomas G.
author_facet Laprell, Laura
Schulze, Christian
Brehme, Marie-Luise
Oertner, Thomas G.
author_sort Laprell, Laura
collection PubMed
description Microglia react to danger signals by rapid and targeted extension of cellular processes towards the source of the signal. This positive chemotactic response is accompanied by a hyperpolarization of the microglia membrane. Here, we show that optogenetic depolarization of microglia has little effect on baseline motility, but significantly slows down the chemotactic response. Reducing the extracellular Ca(2+) concentration mimics the effect of optogenetic depolarization. As the membrane potential sets the driving force for Ca(2+) entry, hyperpolarization is an integral part of rapid stimulus-response coupling in microglia. Compared to typical excitable cells such as neurons, the sign of the activating response is inverted in microglia, leading to inhibition by depolarizing channelrhodopsins. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12974-020-02048-0.
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spelling pubmed-77981952021-01-11 The role of microglia membrane potential in chemotaxis Laprell, Laura Schulze, Christian Brehme, Marie-Luise Oertner, Thomas G. J Neuroinflammation Short Report Microglia react to danger signals by rapid and targeted extension of cellular processes towards the source of the signal. This positive chemotactic response is accompanied by a hyperpolarization of the microglia membrane. Here, we show that optogenetic depolarization of microglia has little effect on baseline motility, but significantly slows down the chemotactic response. Reducing the extracellular Ca(2+) concentration mimics the effect of optogenetic depolarization. As the membrane potential sets the driving force for Ca(2+) entry, hyperpolarization is an integral part of rapid stimulus-response coupling in microglia. Compared to typical excitable cells such as neurons, the sign of the activating response is inverted in microglia, leading to inhibition by depolarizing channelrhodopsins. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12974-020-02048-0. BioMed Central 2021-01-10 /pmc/articles/PMC7798195/ /pubmed/33423699 http://dx.doi.org/10.1186/s12974-020-02048-0 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Laprell, Laura
Schulze, Christian
Brehme, Marie-Luise
Oertner, Thomas G.
The role of microglia membrane potential in chemotaxis
title The role of microglia membrane potential in chemotaxis
title_full The role of microglia membrane potential in chemotaxis
title_fullStr The role of microglia membrane potential in chemotaxis
title_full_unstemmed The role of microglia membrane potential in chemotaxis
title_short The role of microglia membrane potential in chemotaxis
title_sort role of microglia membrane potential in chemotaxis
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798195/
https://www.ncbi.nlm.nih.gov/pubmed/33423699
http://dx.doi.org/10.1186/s12974-020-02048-0
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