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The role of microglia membrane potential in chemotaxis
Microglia react to danger signals by rapid and targeted extension of cellular processes towards the source of the signal. This positive chemotactic response is accompanied by a hyperpolarization of the microglia membrane. Here, we show that optogenetic depolarization of microglia has little effect o...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798195/ https://www.ncbi.nlm.nih.gov/pubmed/33423699 http://dx.doi.org/10.1186/s12974-020-02048-0 |
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author | Laprell, Laura Schulze, Christian Brehme, Marie-Luise Oertner, Thomas G. |
author_facet | Laprell, Laura Schulze, Christian Brehme, Marie-Luise Oertner, Thomas G. |
author_sort | Laprell, Laura |
collection | PubMed |
description | Microglia react to danger signals by rapid and targeted extension of cellular processes towards the source of the signal. This positive chemotactic response is accompanied by a hyperpolarization of the microglia membrane. Here, we show that optogenetic depolarization of microglia has little effect on baseline motility, but significantly slows down the chemotactic response. Reducing the extracellular Ca(2+) concentration mimics the effect of optogenetic depolarization. As the membrane potential sets the driving force for Ca(2+) entry, hyperpolarization is an integral part of rapid stimulus-response coupling in microglia. Compared to typical excitable cells such as neurons, the sign of the activating response is inverted in microglia, leading to inhibition by depolarizing channelrhodopsins. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12974-020-02048-0. |
format | Online Article Text |
id | pubmed-7798195 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-77981952021-01-11 The role of microglia membrane potential in chemotaxis Laprell, Laura Schulze, Christian Brehme, Marie-Luise Oertner, Thomas G. J Neuroinflammation Short Report Microglia react to danger signals by rapid and targeted extension of cellular processes towards the source of the signal. This positive chemotactic response is accompanied by a hyperpolarization of the microglia membrane. Here, we show that optogenetic depolarization of microglia has little effect on baseline motility, but significantly slows down the chemotactic response. Reducing the extracellular Ca(2+) concentration mimics the effect of optogenetic depolarization. As the membrane potential sets the driving force for Ca(2+) entry, hyperpolarization is an integral part of rapid stimulus-response coupling in microglia. Compared to typical excitable cells such as neurons, the sign of the activating response is inverted in microglia, leading to inhibition by depolarizing channelrhodopsins. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12974-020-02048-0. BioMed Central 2021-01-10 /pmc/articles/PMC7798195/ /pubmed/33423699 http://dx.doi.org/10.1186/s12974-020-02048-0 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Short Report Laprell, Laura Schulze, Christian Brehme, Marie-Luise Oertner, Thomas G. The role of microglia membrane potential in chemotaxis |
title | The role of microglia membrane potential in chemotaxis |
title_full | The role of microglia membrane potential in chemotaxis |
title_fullStr | The role of microglia membrane potential in chemotaxis |
title_full_unstemmed | The role of microglia membrane potential in chemotaxis |
title_short | The role of microglia membrane potential in chemotaxis |
title_sort | role of microglia membrane potential in chemotaxis |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798195/ https://www.ncbi.nlm.nih.gov/pubmed/33423699 http://dx.doi.org/10.1186/s12974-020-02048-0 |
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