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Protein over-expression in Escherichia coli triggers adaptation analogous to antimicrobial resistance
BACKGROUND: The E. coli pET system is the most widely used protein over-expression system worldwide. It relies on the assumption that all cells produce target protein and it is generally believed that integral membrane protein (IMP) over-expression is more toxic than their soluble counterparts. RESU...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798265/ https://www.ncbi.nlm.nih.gov/pubmed/33430875 http://dx.doi.org/10.1186/s12934-020-01462-6 |