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Keratin 19 maintains E-cadherin localization at the cell surface and stabilizes cell-cell adhesion of MCF7 cells

A cytoskeletal protein keratin 19 (K19) is highly expressed in breast cancer but its effects on breast cancer cell mechanics are unclear. In MCF7 cells where K19 expression is ablated,we found that K19 is required to maintain rounded epithelial-like shape and tight cell-cell adhesion. A loss of K19...

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Autores principales: Alsharif, Sarah, Sharma, Pooja, Bursch, Karina, Milliken, Rachel, Lam, Van, Fallatah, Arwa, Phan, Thuc, Collins, Meagan, Dohlman, Priya, Tiufekchiev, Sarah, Nehmetallah, Georges, Raub, Christopher B., Chung, Byung Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801129/
https://www.ncbi.nlm.nih.gov/pubmed/33393839
http://dx.doi.org/10.1080/19336918.2020.1868694
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author Alsharif, Sarah
Sharma, Pooja
Bursch, Karina
Milliken, Rachel
Lam, Van
Fallatah, Arwa
Phan, Thuc
Collins, Meagan
Dohlman, Priya
Tiufekchiev, Sarah
Nehmetallah, Georges
Raub, Christopher B.
Chung, Byung Min
author_facet Alsharif, Sarah
Sharma, Pooja
Bursch, Karina
Milliken, Rachel
Lam, Van
Fallatah, Arwa
Phan, Thuc
Collins, Meagan
Dohlman, Priya
Tiufekchiev, Sarah
Nehmetallah, Georges
Raub, Christopher B.
Chung, Byung Min
author_sort Alsharif, Sarah
collection PubMed
description A cytoskeletal protein keratin 19 (K19) is highly expressed in breast cancer but its effects on breast cancer cell mechanics are unclear. In MCF7 cells where K19 expression is ablated,we found that K19 is required to maintain rounded epithelial-like shape and tight cell-cell adhesion. A loss of K19 also lowered cell surface E-cadherin levels. Inhibiting internalization restored cell-cell adhesion of KRT19 knockout cells, suggesting that E-cadherin internalization contributed to defective adhesion. Ultimately, while K19 inhibited cell migration and invasion, it was required for cells to form colonies in suspension. Our results suggest that K19 stabilizes E-cadherin complexes at the cell membrane to maintain cell-cell adhesion which inhibits cell invasiveness but provides growth and survival advantages for circulating tumor cells.
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spelling pubmed-78011292021-01-21 Keratin 19 maintains E-cadherin localization at the cell surface and stabilizes cell-cell adhesion of MCF7 cells Alsharif, Sarah Sharma, Pooja Bursch, Karina Milliken, Rachel Lam, Van Fallatah, Arwa Phan, Thuc Collins, Meagan Dohlman, Priya Tiufekchiev, Sarah Nehmetallah, Georges Raub, Christopher B. Chung, Byung Min Cell Adh Migr Research Paper A cytoskeletal protein keratin 19 (K19) is highly expressed in breast cancer but its effects on breast cancer cell mechanics are unclear. In MCF7 cells where K19 expression is ablated,we found that K19 is required to maintain rounded epithelial-like shape and tight cell-cell adhesion. A loss of K19 also lowered cell surface E-cadherin levels. Inhibiting internalization restored cell-cell adhesion of KRT19 knockout cells, suggesting that E-cadherin internalization contributed to defective adhesion. Ultimately, while K19 inhibited cell migration and invasion, it was required for cells to form colonies in suspension. Our results suggest that K19 stabilizes E-cadherin complexes at the cell membrane to maintain cell-cell adhesion which inhibits cell invasiveness but provides growth and survival advantages for circulating tumor cells. Taylor & Francis 2021-01-04 /pmc/articles/PMC7801129/ /pubmed/33393839 http://dx.doi.org/10.1080/19336918.2020.1868694 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Alsharif, Sarah
Sharma, Pooja
Bursch, Karina
Milliken, Rachel
Lam, Van
Fallatah, Arwa
Phan, Thuc
Collins, Meagan
Dohlman, Priya
Tiufekchiev, Sarah
Nehmetallah, Georges
Raub, Christopher B.
Chung, Byung Min
Keratin 19 maintains E-cadherin localization at the cell surface and stabilizes cell-cell adhesion of MCF7 cells
title Keratin 19 maintains E-cadherin localization at the cell surface and stabilizes cell-cell adhesion of MCF7 cells
title_full Keratin 19 maintains E-cadherin localization at the cell surface and stabilizes cell-cell adhesion of MCF7 cells
title_fullStr Keratin 19 maintains E-cadherin localization at the cell surface and stabilizes cell-cell adhesion of MCF7 cells
title_full_unstemmed Keratin 19 maintains E-cadherin localization at the cell surface and stabilizes cell-cell adhesion of MCF7 cells
title_short Keratin 19 maintains E-cadherin localization at the cell surface and stabilizes cell-cell adhesion of MCF7 cells
title_sort keratin 19 maintains e-cadherin localization at the cell surface and stabilizes cell-cell adhesion of mcf7 cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801129/
https://www.ncbi.nlm.nih.gov/pubmed/33393839
http://dx.doi.org/10.1080/19336918.2020.1868694
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