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Keratin 19 maintains E-cadherin localization at the cell surface and stabilizes cell-cell adhesion of MCF7 cells
A cytoskeletal protein keratin 19 (K19) is highly expressed in breast cancer but its effects on breast cancer cell mechanics are unclear. In MCF7 cells where K19 expression is ablated,we found that K19 is required to maintain rounded epithelial-like shape and tight cell-cell adhesion. A loss of K19...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801129/ https://www.ncbi.nlm.nih.gov/pubmed/33393839 http://dx.doi.org/10.1080/19336918.2020.1868694 |
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author | Alsharif, Sarah Sharma, Pooja Bursch, Karina Milliken, Rachel Lam, Van Fallatah, Arwa Phan, Thuc Collins, Meagan Dohlman, Priya Tiufekchiev, Sarah Nehmetallah, Georges Raub, Christopher B. Chung, Byung Min |
author_facet | Alsharif, Sarah Sharma, Pooja Bursch, Karina Milliken, Rachel Lam, Van Fallatah, Arwa Phan, Thuc Collins, Meagan Dohlman, Priya Tiufekchiev, Sarah Nehmetallah, Georges Raub, Christopher B. Chung, Byung Min |
author_sort | Alsharif, Sarah |
collection | PubMed |
description | A cytoskeletal protein keratin 19 (K19) is highly expressed in breast cancer but its effects on breast cancer cell mechanics are unclear. In MCF7 cells where K19 expression is ablated,we found that K19 is required to maintain rounded epithelial-like shape and tight cell-cell adhesion. A loss of K19 also lowered cell surface E-cadherin levels. Inhibiting internalization restored cell-cell adhesion of KRT19 knockout cells, suggesting that E-cadherin internalization contributed to defective adhesion. Ultimately, while K19 inhibited cell migration and invasion, it was required for cells to form colonies in suspension. Our results suggest that K19 stabilizes E-cadherin complexes at the cell membrane to maintain cell-cell adhesion which inhibits cell invasiveness but provides growth and survival advantages for circulating tumor cells. |
format | Online Article Text |
id | pubmed-7801129 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-78011292021-01-21 Keratin 19 maintains E-cadherin localization at the cell surface and stabilizes cell-cell adhesion of MCF7 cells Alsharif, Sarah Sharma, Pooja Bursch, Karina Milliken, Rachel Lam, Van Fallatah, Arwa Phan, Thuc Collins, Meagan Dohlman, Priya Tiufekchiev, Sarah Nehmetallah, Georges Raub, Christopher B. Chung, Byung Min Cell Adh Migr Research Paper A cytoskeletal protein keratin 19 (K19) is highly expressed in breast cancer but its effects on breast cancer cell mechanics are unclear. In MCF7 cells where K19 expression is ablated,we found that K19 is required to maintain rounded epithelial-like shape and tight cell-cell adhesion. A loss of K19 also lowered cell surface E-cadherin levels. Inhibiting internalization restored cell-cell adhesion of KRT19 knockout cells, suggesting that E-cadherin internalization contributed to defective adhesion. Ultimately, while K19 inhibited cell migration and invasion, it was required for cells to form colonies in suspension. Our results suggest that K19 stabilizes E-cadherin complexes at the cell membrane to maintain cell-cell adhesion which inhibits cell invasiveness but provides growth and survival advantages for circulating tumor cells. Taylor & Francis 2021-01-04 /pmc/articles/PMC7801129/ /pubmed/33393839 http://dx.doi.org/10.1080/19336918.2020.1868694 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Alsharif, Sarah Sharma, Pooja Bursch, Karina Milliken, Rachel Lam, Van Fallatah, Arwa Phan, Thuc Collins, Meagan Dohlman, Priya Tiufekchiev, Sarah Nehmetallah, Georges Raub, Christopher B. Chung, Byung Min Keratin 19 maintains E-cadherin localization at the cell surface and stabilizes cell-cell adhesion of MCF7 cells |
title | Keratin 19 maintains E-cadherin localization at the cell surface and stabilizes cell-cell adhesion of MCF7 cells |
title_full | Keratin 19 maintains E-cadherin localization at the cell surface and stabilizes cell-cell adhesion of MCF7 cells |
title_fullStr | Keratin 19 maintains E-cadherin localization at the cell surface and stabilizes cell-cell adhesion of MCF7 cells |
title_full_unstemmed | Keratin 19 maintains E-cadherin localization at the cell surface and stabilizes cell-cell adhesion of MCF7 cells |
title_short | Keratin 19 maintains E-cadherin localization at the cell surface and stabilizes cell-cell adhesion of MCF7 cells |
title_sort | keratin 19 maintains e-cadherin localization at the cell surface and stabilizes cell-cell adhesion of mcf7 cells |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801129/ https://www.ncbi.nlm.nih.gov/pubmed/33393839 http://dx.doi.org/10.1080/19336918.2020.1868694 |
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