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Preoperative detection of KRAS mutated circulating tumor DNA is an independent risk factor for recurrence in colorectal cancer

Preoperative ctDNA status in relation to recurrence in cases of CRC remains unclear. We examined preoperative ctDNA detection by targeting KRAS gene mutations as a predictive marker for recurrence after CRC surgery. We measured the preoperative KRAS mutated ctDNA status and analyzed the correlation...

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Autores principales: Nakamura, Yuki, Yokoyama, Shozo, Matsuda, Kenji, Tamura, Koichi, Mitani, Yasuyuki, Iwamoto, Hiromitsu, Mizumoto, Yuki, Murakami, Daisuke, Kitahata, Yuji, Yamaue, Hiroki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801374/
https://www.ncbi.nlm.nih.gov/pubmed/33432066
http://dx.doi.org/10.1038/s41598-020-79909-4
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author Nakamura, Yuki
Yokoyama, Shozo
Matsuda, Kenji
Tamura, Koichi
Mitani, Yasuyuki
Iwamoto, Hiromitsu
Mizumoto, Yuki
Murakami, Daisuke
Kitahata, Yuji
Yamaue, Hiroki
author_facet Nakamura, Yuki
Yokoyama, Shozo
Matsuda, Kenji
Tamura, Koichi
Mitani, Yasuyuki
Iwamoto, Hiromitsu
Mizumoto, Yuki
Murakami, Daisuke
Kitahata, Yuji
Yamaue, Hiroki
author_sort Nakamura, Yuki
collection PubMed
description Preoperative ctDNA status in relation to recurrence in cases of CRC remains unclear. We examined preoperative ctDNA detection by targeting KRAS gene mutations as a predictive marker for recurrence after CRC surgery. We measured the preoperative KRAS mutated ctDNA status and analyzed the correlation with clinicopathologic features of 180 patients that underwent surgery for CRC. We studied the association between preoperative KRAS mutated ctDNA and postoperative recurrence in patients (n = 150) that underwent radical surgery. KRAS mutated ctDNA was detected in 59 patients (32.8%). Median mutant allele frequency of KRAS in ctDNA was 0.20%. KRAS status in ctDNA and lymph node metastasis and distant metastasis were not significantly different. Among patients that underwent radical resection, recurrence occurred in 21 (14.0%, median follow-up 24 months). In Kaplan–Meier analysis, preoperative detection of KRAS mutated ctDNA was associated with inferior recurrence-free interval (RFI) (p = 0.002) and recurrence-free survival (RFS) (p = 0.025). In a multivariate Cox proportional hazards model, preoperative detection of KRAS mutated ctDNA was an independent factor related to both RFI (HR = 3.08; p = 0.012) and RFS (HR = 2.18; p = 0.044). Preoperative measurement of KRAS mutated ctDNA could be useful to decide postoperative treatment.
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spelling pubmed-78013742021-01-12 Preoperative detection of KRAS mutated circulating tumor DNA is an independent risk factor for recurrence in colorectal cancer Nakamura, Yuki Yokoyama, Shozo Matsuda, Kenji Tamura, Koichi Mitani, Yasuyuki Iwamoto, Hiromitsu Mizumoto, Yuki Murakami, Daisuke Kitahata, Yuji Yamaue, Hiroki Sci Rep Article Preoperative ctDNA status in relation to recurrence in cases of CRC remains unclear. We examined preoperative ctDNA detection by targeting KRAS gene mutations as a predictive marker for recurrence after CRC surgery. We measured the preoperative KRAS mutated ctDNA status and analyzed the correlation with clinicopathologic features of 180 patients that underwent surgery for CRC. We studied the association between preoperative KRAS mutated ctDNA and postoperative recurrence in patients (n = 150) that underwent radical surgery. KRAS mutated ctDNA was detected in 59 patients (32.8%). Median mutant allele frequency of KRAS in ctDNA was 0.20%. KRAS status in ctDNA and lymph node metastasis and distant metastasis were not significantly different. Among patients that underwent radical resection, recurrence occurred in 21 (14.0%, median follow-up 24 months). In Kaplan–Meier analysis, preoperative detection of KRAS mutated ctDNA was associated with inferior recurrence-free interval (RFI) (p = 0.002) and recurrence-free survival (RFS) (p = 0.025). In a multivariate Cox proportional hazards model, preoperative detection of KRAS mutated ctDNA was an independent factor related to both RFI (HR = 3.08; p = 0.012) and RFS (HR = 2.18; p = 0.044). Preoperative measurement of KRAS mutated ctDNA could be useful to decide postoperative treatment. Nature Publishing Group UK 2021-01-11 /pmc/articles/PMC7801374/ /pubmed/33432066 http://dx.doi.org/10.1038/s41598-020-79909-4 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Nakamura, Yuki
Yokoyama, Shozo
Matsuda, Kenji
Tamura, Koichi
Mitani, Yasuyuki
Iwamoto, Hiromitsu
Mizumoto, Yuki
Murakami, Daisuke
Kitahata, Yuji
Yamaue, Hiroki
Preoperative detection of KRAS mutated circulating tumor DNA is an independent risk factor for recurrence in colorectal cancer
title Preoperative detection of KRAS mutated circulating tumor DNA is an independent risk factor for recurrence in colorectal cancer
title_full Preoperative detection of KRAS mutated circulating tumor DNA is an independent risk factor for recurrence in colorectal cancer
title_fullStr Preoperative detection of KRAS mutated circulating tumor DNA is an independent risk factor for recurrence in colorectal cancer
title_full_unstemmed Preoperative detection of KRAS mutated circulating tumor DNA is an independent risk factor for recurrence in colorectal cancer
title_short Preoperative detection of KRAS mutated circulating tumor DNA is an independent risk factor for recurrence in colorectal cancer
title_sort preoperative detection of kras mutated circulating tumor dna is an independent risk factor for recurrence in colorectal cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801374/
https://www.ncbi.nlm.nih.gov/pubmed/33432066
http://dx.doi.org/10.1038/s41598-020-79909-4
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