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NGS-PrimerPlex: High-throughput primer design for multiplex polymerase chain reactions

Multiplex polymerase chain reaction (PCR) has multiple applications in molecular biology, including developing new targeted next-generation sequencing (NGS) panels. We present NGS-PrimerPlex, an efficient and versatile command-line application that designs primers for different refined types of ampl...

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Detalles Bibliográficos
Autores principales: Kechin, Andrey, Borobova, Viktoria, Boyarskikh, Ulyana, Khrapov, Evgeniy, Subbotin, Sergey, Filipenko, Maxim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802936/
https://www.ncbi.nlm.nih.gov/pubmed/33378360
http://dx.doi.org/10.1371/journal.pcbi.1008468