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NGS-PrimerPlex: High-throughput primer design for multiplex polymerase chain reactions
Multiplex polymerase chain reaction (PCR) has multiple applications in molecular biology, including developing new targeted next-generation sequencing (NGS) panels. We present NGS-PrimerPlex, an efficient and versatile command-line application that designs primers for different refined types of ampl...
Autores principales: | Kechin, Andrey, Borobova, Viktoria, Boyarskikh, Ulyana, Khrapov, Evgeniy, Subbotin, Sergey, Filipenko, Maxim |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802936/ https://www.ncbi.nlm.nih.gov/pubmed/33378360 http://dx.doi.org/10.1371/journal.pcbi.1008468 |
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