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Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing
We xeno-transplanted human neural precursor cells derived from induced pluripotent stem cells into the cerebellum and brainstem of mice and rats during prenatal development or the first postnatal week. The transplants survived and started to differentiate up to 1 month after birth when they were rej...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7803978/ https://www.ncbi.nlm.nih.gov/pubmed/33436685 http://dx.doi.org/10.1038/s41598-020-79502-9 |
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author | Nato, Giulia Corti, Alessandro Parmigiani, Elena Jachetti, Elena Lecis, Daniele Colombo, Mario Paolo Delia, Domenico Buffo, Annalisa Magrassi, Lorenzo |
author_facet | Nato, Giulia Corti, Alessandro Parmigiani, Elena Jachetti, Elena Lecis, Daniele Colombo, Mario Paolo Delia, Domenico Buffo, Annalisa Magrassi, Lorenzo |
author_sort | Nato, Giulia |
collection | PubMed |
description | We xeno-transplanted human neural precursor cells derived from induced pluripotent stem cells into the cerebellum and brainstem of mice and rats during prenatal development or the first postnatal week. The transplants survived and started to differentiate up to 1 month after birth when they were rejected by both species. Extended survival and differentiation of the same cells were obtained only when they were transplanted in NOD-SCID mice. Transplants of human neural precursor cells mixed with the same cells after partial in vitro differentiation or with a cellular extract obtained from adult rat cerebellum increased survival of the xeno-graft beyond one month. These findings are consistent with the hypothesis that the slower pace of differentiation of human neural precursors compared to that of rodents restricts induction of immune-tolerance to human antigens expressed before completion of maturation of the immune system. With further maturation the transplanted neural precursors expressed more mature antigens before the graft were rejected. Supplementation of the immature cells suspensions with more mature antigens may help to induce immune-tolerance for those antigens expressed only later by the engrafted cells. |
format | Online Article Text |
id | pubmed-7803978 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78039782021-01-13 Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing Nato, Giulia Corti, Alessandro Parmigiani, Elena Jachetti, Elena Lecis, Daniele Colombo, Mario Paolo Delia, Domenico Buffo, Annalisa Magrassi, Lorenzo Sci Rep Article We xeno-transplanted human neural precursor cells derived from induced pluripotent stem cells into the cerebellum and brainstem of mice and rats during prenatal development or the first postnatal week. The transplants survived and started to differentiate up to 1 month after birth when they were rejected by both species. Extended survival and differentiation of the same cells were obtained only when they were transplanted in NOD-SCID mice. Transplants of human neural precursor cells mixed with the same cells after partial in vitro differentiation or with a cellular extract obtained from adult rat cerebellum increased survival of the xeno-graft beyond one month. These findings are consistent with the hypothesis that the slower pace of differentiation of human neural precursors compared to that of rodents restricts induction of immune-tolerance to human antigens expressed before completion of maturation of the immune system. With further maturation the transplanted neural precursors expressed more mature antigens before the graft were rejected. Supplementation of the immature cells suspensions with more mature antigens may help to induce immune-tolerance for those antigens expressed only later by the engrafted cells. Nature Publishing Group UK 2021-01-12 /pmc/articles/PMC7803978/ /pubmed/33436685 http://dx.doi.org/10.1038/s41598-020-79502-9 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Nato, Giulia Corti, Alessandro Parmigiani, Elena Jachetti, Elena Lecis, Daniele Colombo, Mario Paolo Delia, Domenico Buffo, Annalisa Magrassi, Lorenzo Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing |
title | Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing |
title_full | Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing |
title_fullStr | Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing |
title_full_unstemmed | Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing |
title_short | Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing |
title_sort | immune-tolerance to human ips-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7803978/ https://www.ncbi.nlm.nih.gov/pubmed/33436685 http://dx.doi.org/10.1038/s41598-020-79502-9 |
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