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A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines

Aedes (Ae.) aegypti and Ae. albopictus mosquitoes transmit arthropod-borne diseases around the globe, causing ~ 700,000 deaths each year. Genetic mutants are valuable tools to interrogate both fundamental vector biology and mosquito host factors important for viral infection. However, very few genet...

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Detalles Bibliográficos
Autores principales: Rozen-Gagnon, Kathryn, Yi, Soon, Jacobson, Eliana, Novack, Sasha, Rice, Charles M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7804293/
https://www.ncbi.nlm.nih.gov/pubmed/33436886
http://dx.doi.org/10.1038/s41598-020-80436-5