An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells

In T cell-based cancer immunotherapy, tumor antigen (Ag)-specific CD8(+) cytotoxic T lymphocytes (CTLs) can specifically target tumor Ags on malignant cells. This promising approach drove us to adopt this strategy of T cell transfer (ACT)-based immunotherapy for chronic viral infections. Here, we de...

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Detalles Bibliográficos
Autores principales: Haque, Mohammad, Xiong, Xiaofang, Lei, Fengyang, Das, Jugal Kishore, Song, Jianxun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806515/
https://www.ncbi.nlm.nih.gov/pubmed/33490980
http://dx.doi.org/10.1016/j.xpro.2020.100264
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author Haque, Mohammad
Xiong, Xiaofang
Lei, Fengyang
Das, Jugal Kishore
Song, Jianxun
author_facet Haque, Mohammad
Xiong, Xiaofang
Lei, Fengyang
Das, Jugal Kishore
Song, Jianxun
author_sort Haque, Mohammad
collection PubMed
description In T cell-based cancer immunotherapy, tumor antigen (Ag)-specific CD8(+) cytotoxic T lymphocytes (CTLs) can specifically target tumor Ags on malignant cells. This promising approach drove us to adopt this strategy of T cell transfer (ACT)-based immunotherapy for chronic viral infections. Here, we describe the generation of hepatitis B virus (HBV) Ag-specific CTLs from induced pluripotent stem cells (iPSCs), i.e., iPSC-CTLs. Ag-specific iPSC-CTLs can target HBV Ag(+) cells and infiltrate into the liver to suppress HBV replication in a murine model. For complete details on the use and execution of this protocol, please refer to Haque et al. (2020).
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spelling pubmed-78065152021-01-22 An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells Haque, Mohammad Xiong, Xiaofang Lei, Fengyang Das, Jugal Kishore Song, Jianxun STAR Protoc Protocol In T cell-based cancer immunotherapy, tumor antigen (Ag)-specific CD8(+) cytotoxic T lymphocytes (CTLs) can specifically target tumor Ags on malignant cells. This promising approach drove us to adopt this strategy of T cell transfer (ACT)-based immunotherapy for chronic viral infections. Here, we describe the generation of hepatitis B virus (HBV) Ag-specific CTLs from induced pluripotent stem cells (iPSCs), i.e., iPSC-CTLs. Ag-specific iPSC-CTLs can target HBV Ag(+) cells and infiltrate into the liver to suppress HBV replication in a murine model. For complete details on the use and execution of this protocol, please refer to Haque et al. (2020). Elsevier 2021-01-12 /pmc/articles/PMC7806515/ /pubmed/33490980 http://dx.doi.org/10.1016/j.xpro.2020.100264 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Haque, Mohammad
Xiong, Xiaofang
Lei, Fengyang
Das, Jugal Kishore
Song, Jianxun
An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells
title An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells
title_full An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells
title_fullStr An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells
title_full_unstemmed An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells
title_short An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells
title_sort optimized protocol for the generation of hbv viral antigen-specific t lymphocytes from pluripotent stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806515/
https://www.ncbi.nlm.nih.gov/pubmed/33490980
http://dx.doi.org/10.1016/j.xpro.2020.100264
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