Differential effects on human cytochromes P450 by CRISPR/Cas9-induced genetic knockout of cytochrome P450 reductase and cytochrome b5 in HepaRG cells

HepaRG cells are increasingly accepted as model for human drug metabolism and other hepatic functions. We used lentiviral transduction of undifferentiated HepaRG cells to deliver Cas9 and two alternative sgRNAs targeted at NADPH:cytochrome P450 oxidoreductase (POR), the obligate electron donor for m...

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Detalles Bibliográficos
Autores principales: Heintze, Tamara, Klein, Kathrin, Hofmann, Ute, Zanger, Ulrich M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806635/
https://www.ncbi.nlm.nih.gov/pubmed/33441761
http://dx.doi.org/10.1038/s41598-020-79952-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806635/
https://www.ncbi.nlm.nih.gov/pubmed/33441761
http://dx.doi.org/10.1038/s41598-020-79952-1

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