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An absorbance method for analysis of enzymatic degradation kinetics of poly(ethylene terephthalate) films

Increased interest in poly(ethylene terephthalate) (PET)-degrading enzymes (PETases) have generated efforts to find mutants with improved catalytic activity and thermostability. Here, we present a simple and fast method to determine relative enzyme kinetics through bulk absorbance measurements of re...

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Detalles Bibliográficos
Autores principales: Zhong-Johnson, En Ze Linda, Voigt, Christopher A., Sinskey, Anthony J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806724/
https://www.ncbi.nlm.nih.gov/pubmed/33441590
http://dx.doi.org/10.1038/s41598-020-79031-5