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Partial Characterization of an Anti-Listerial Bacteriocin from Enterococcus faecium CJNU 2524

Listeria monocytogenes is a representative foodborne pathogen and causes listeriosis. Enterococcus faecium CJNU 2524 was confirmed to produce a bacteriocin with anti-listerial activity. To establish optimal culture conditions for the production of the bacteriocin from E. faecium CJNU 2524, different...

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Autores principales: Yang, Jung-Mo, Moon, Gi-Seong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Food Science of Animal Resources 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810390/
https://www.ncbi.nlm.nih.gov/pubmed/33506226
http://dx.doi.org/10.5851/kosfa.2020.e98
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author Yang, Jung-Mo
Moon, Gi-Seong
author_facet Yang, Jung-Mo
Moon, Gi-Seong
author_sort Yang, Jung-Mo
collection PubMed
description Listeria monocytogenes is a representative foodborne pathogen and causes listeriosis. Enterococcus faecium CJNU 2524 was confirmed to produce a bacteriocin with anti-listerial activity. To establish optimal culture conditions for the production of the bacteriocin from E. faecium CJNU 2524, different media (MRS and BHI broth) and temperatures (25°C, 30°C, and 37°C) were investigated. The results showed that the optimal culture conditions were MRS broth and 25°C or 30°C temperatures. The crude bacteriocin was stable in a broad range of pH conditions (2.0–10.0), temperatures (60°C–100°C), and organic solvents (methanol, ethanol, acetone, acetonitrile, and chloroform). The bacteriocin activity was abolished when treated with protease but not α-amylase or lipase, indicating the proteinaceous nature of the bacteriocin. Finally, the bacteriocin showed a bactericidal mode of action against L. monocytogenes. Therefore, it can be a biopreservative candidate for controlling L. monocytogenes in dairy and meat products.
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spelling pubmed-78103902021-01-26 Partial Characterization of an Anti-Listerial Bacteriocin from Enterococcus faecium CJNU 2524 Yang, Jung-Mo Moon, Gi-Seong Food Sci Anim Resour Short Communication Listeria monocytogenes is a representative foodborne pathogen and causes listeriosis. Enterococcus faecium CJNU 2524 was confirmed to produce a bacteriocin with anti-listerial activity. To establish optimal culture conditions for the production of the bacteriocin from E. faecium CJNU 2524, different media (MRS and BHI broth) and temperatures (25°C, 30°C, and 37°C) were investigated. The results showed that the optimal culture conditions were MRS broth and 25°C or 30°C temperatures. The crude bacteriocin was stable in a broad range of pH conditions (2.0–10.0), temperatures (60°C–100°C), and organic solvents (methanol, ethanol, acetone, acetonitrile, and chloroform). The bacteriocin activity was abolished when treated with protease but not α-amylase or lipase, indicating the proteinaceous nature of the bacteriocin. Finally, the bacteriocin showed a bactericidal mode of action against L. monocytogenes. Therefore, it can be a biopreservative candidate for controlling L. monocytogenes in dairy and meat products. Korean Society for Food Science of Animal Resources 2021-01 2021-01-01 /pmc/articles/PMC7810390/ /pubmed/33506226 http://dx.doi.org/10.5851/kosfa.2020.e98 Text en © Korean Society for Food Science of Animal Resources http://creativecommons.org/licenses/by-nc/3.0/ This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Yang, Jung-Mo
Moon, Gi-Seong
Partial Characterization of an Anti-Listerial Bacteriocin from Enterococcus faecium CJNU 2524
title Partial Characterization of an Anti-Listerial Bacteriocin from Enterococcus faecium CJNU 2524
title_full Partial Characterization of an Anti-Listerial Bacteriocin from Enterococcus faecium CJNU 2524
title_fullStr Partial Characterization of an Anti-Listerial Bacteriocin from Enterococcus faecium CJNU 2524
title_full_unstemmed Partial Characterization of an Anti-Listerial Bacteriocin from Enterococcus faecium CJNU 2524
title_short Partial Characterization of an Anti-Listerial Bacteriocin from Enterococcus faecium CJNU 2524
title_sort partial characterization of an anti-listerial bacteriocin from enterococcus faecium cjnu 2524
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810390/
https://www.ncbi.nlm.nih.gov/pubmed/33506226
http://dx.doi.org/10.5851/kosfa.2020.e98
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