Affinity enrichment and identification of inositol poly- and pyrophosphate interactomes

This protocol describes an affinity enrichment approach from mammalian cell extracts to identify protein binding partners of inositol hexakisphosphate (InsP(6)) and 5-diphosphoinositol pentakisphosphate (5PP-InsP(5)), two important eukaryotic metabolites. The interactomes are annotated using mass sp...

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Detalles Bibliográficos
Autores principales: Furkert, David, Nadler-Holly, Michal, Fiedler, Dorothea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811051/
https://www.ncbi.nlm.nih.gov/pubmed/33490990
http://dx.doi.org/10.1016/j.xpro.2020.100277

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811051/
https://www.ncbi.nlm.nih.gov/pubmed/33490990
http://dx.doi.org/10.1016/j.xpro.2020.100277

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