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Affinity enrichment and identification of inositol poly- and pyrophosphate interactomes
This protocol describes an affinity enrichment approach from mammalian cell extracts to identify protein binding partners of inositol hexakisphosphate (InsP(6)) and 5-diphosphoinositol pentakisphosphate (5PP-InsP(5)), two important eukaryotic metabolites. The interactomes are annotated using mass sp...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811051/ https://www.ncbi.nlm.nih.gov/pubmed/33490990 http://dx.doi.org/10.1016/j.xpro.2020.100277 |