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Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting

Cardiac exophers are membrane-bound extracellular vesicles released by cardiomyocytes with varied content and an average diameter of 3.5 μm. Here, we provide a detailed protocol to enable the identification and purification of cardiomyocyte-derived exophers by using fluorescence-activated cell sorti...

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Detalles Bibliográficos
Autores principales: Nicolás-Ávila, José Ángel, Sánchez-Diaz, María, Hidalgo, Andrés
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811054/
https://www.ncbi.nlm.nih.gov/pubmed/33490991
http://dx.doi.org/10.1016/j.xpro.2020.100286