Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting

Cardiac exophers are membrane-bound extracellular vesicles released by cardiomyocytes with varied content and an average diameter of 3.5 μm. Here, we provide a detailed protocol to enable the identification and purification of cardiomyocyte-derived exophers by using fluorescence-activated cell sorti...

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Detalles Bibliográficos
Autores principales: Nicolás-Ávila, José Ángel, Sánchez-Diaz, María, Hidalgo, Andrés
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811054/
https://www.ncbi.nlm.nih.gov/pubmed/33490991
http://dx.doi.org/10.1016/j.xpro.2020.100286
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author Nicolás-Ávila, José Ángel
Sánchez-Diaz, María
Hidalgo, Andrés
author_facet Nicolás-Ávila, José Ángel
Sánchez-Diaz, María
Hidalgo, Andrés
author_sort Nicolás-Ávila, José Ángel
collection PubMed
description Cardiac exophers are membrane-bound extracellular vesicles released by cardiomyocytes with varied content and an average diameter of 3.5 μm. Here, we provide a detailed protocol to enable the identification and purification of cardiomyocyte-derived exophers by using fluorescence-activated cell sorting for downstream cellular and molecular analysis. This protocol requires the use of mouse strains expressing fluorescent proteins in cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Nicolás-Ávila et al. (2020).
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spelling pubmed-78110542021-01-22 Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting Nicolás-Ávila, José Ángel Sánchez-Diaz, María Hidalgo, Andrés STAR Protoc Protocol Cardiac exophers are membrane-bound extracellular vesicles released by cardiomyocytes with varied content and an average diameter of 3.5 μm. Here, we provide a detailed protocol to enable the identification and purification of cardiomyocyte-derived exophers by using fluorescence-activated cell sorting for downstream cellular and molecular analysis. This protocol requires the use of mouse strains expressing fluorescent proteins in cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Nicolás-Ávila et al. (2020). Elsevier 2021-01-14 /pmc/articles/PMC7811054/ /pubmed/33490991 http://dx.doi.org/10.1016/j.xpro.2020.100286 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Nicolás-Ávila, José Ángel
Sánchez-Diaz, María
Hidalgo, Andrés
Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting
title Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting
title_full Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting
title_fullStr Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting
title_full_unstemmed Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting
title_short Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting
title_sort isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811054/
https://www.ncbi.nlm.nih.gov/pubmed/33490991
http://dx.doi.org/10.1016/j.xpro.2020.100286
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