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Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting
Cardiac exophers are membrane-bound extracellular vesicles released by cardiomyocytes with varied content and an average diameter of 3.5 μm. Here, we provide a detailed protocol to enable the identification and purification of cardiomyocyte-derived exophers by using fluorescence-activated cell sorti...
Autores principales: | Nicolás-Ávila, José Ángel, Sánchez-Diaz, María, Hidalgo, Andrés |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811054/ https://www.ncbi.nlm.nih.gov/pubmed/33490991 http://dx.doi.org/10.1016/j.xpro.2020.100286 |
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