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Ubiquitin‐specific protease 2 regulates Ang Ⅱ–induced cardiac fibroblasts activation by up‐regulating cyclin D1 and stabilizing β‐catenin in vitro

Cardiac fibrosis, featuring abnormally elevated extracellular matrix accumulation, decreases tissue compliance, impairs cardiac function and accelerates heart failure. Mounting evidence suggests that the ubiquitin proteasome pathway is involved in cardiac fibrosis. In the present study, ubiquitin‐sp...

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Autores principales: Xu, Qiong, Liu, Mingke, Zhang, Fangcheng, Liu, Xiaolin, Ling, Sisi, Chen, Xuke, Gu, Jielei, Ou, Wenchao, Liu, Shiming, Liu, Ningning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812274/
https://www.ncbi.nlm.nih.gov/pubmed/33314748
http://dx.doi.org/10.1111/jcmm.16162
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author Xu, Qiong
Liu, Mingke
Zhang, Fangcheng
Liu, Xiaolin
Ling, Sisi
Chen, Xuke
Gu, Jielei
Ou, Wenchao
Liu, Shiming
Liu, Ningning
author_facet Xu, Qiong
Liu, Mingke
Zhang, Fangcheng
Liu, Xiaolin
Ling, Sisi
Chen, Xuke
Gu, Jielei
Ou, Wenchao
Liu, Shiming
Liu, Ningning
author_sort Xu, Qiong
collection PubMed
description Cardiac fibrosis, featuring abnormally elevated extracellular matrix accumulation, decreases tissue compliance, impairs cardiac function and accelerates heart failure. Mounting evidence suggests that the ubiquitin proteasome pathway is involved in cardiac fibrosis. In the present study, ubiquitin‐specific protease 2 (USP2) was identified as a novel therapeutic target in cardiac fibrosis. Indeed, USP2 expression was increased in angiotensin II–induced primary cardiac fibroblasts (CFs) from neonatal rats. In addition, USP2 inhibition suppressed CFs proliferation, collagen synthesis and cell cycle progression. Furthermore, USP2 interacted with β‐catenin, thereby regulating its deubiquitination and stabilization in CFs. To sum up, these findings revealed that USP2 has a therapeutic potential for the treatment of cardiac fibrosis.
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spelling pubmed-78122742021-01-22 Ubiquitin‐specific protease 2 regulates Ang Ⅱ–induced cardiac fibroblasts activation by up‐regulating cyclin D1 and stabilizing β‐catenin in vitro Xu, Qiong Liu, Mingke Zhang, Fangcheng Liu, Xiaolin Ling, Sisi Chen, Xuke Gu, Jielei Ou, Wenchao Liu, Shiming Liu, Ningning J Cell Mol Med Original Articles Cardiac fibrosis, featuring abnormally elevated extracellular matrix accumulation, decreases tissue compliance, impairs cardiac function and accelerates heart failure. Mounting evidence suggests that the ubiquitin proteasome pathway is involved in cardiac fibrosis. In the present study, ubiquitin‐specific protease 2 (USP2) was identified as a novel therapeutic target in cardiac fibrosis. Indeed, USP2 expression was increased in angiotensin II–induced primary cardiac fibroblasts (CFs) from neonatal rats. In addition, USP2 inhibition suppressed CFs proliferation, collagen synthesis and cell cycle progression. Furthermore, USP2 interacted with β‐catenin, thereby regulating its deubiquitination and stabilization in CFs. To sum up, these findings revealed that USP2 has a therapeutic potential for the treatment of cardiac fibrosis. John Wiley and Sons Inc. 2020-12-12 2021-01 /pmc/articles/PMC7812274/ /pubmed/33314748 http://dx.doi.org/10.1111/jcmm.16162 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Xu, Qiong
Liu, Mingke
Zhang, Fangcheng
Liu, Xiaolin
Ling, Sisi
Chen, Xuke
Gu, Jielei
Ou, Wenchao
Liu, Shiming
Liu, Ningning
Ubiquitin‐specific protease 2 regulates Ang Ⅱ–induced cardiac fibroblasts activation by up‐regulating cyclin D1 and stabilizing β‐catenin in vitro
title Ubiquitin‐specific protease 2 regulates Ang Ⅱ–induced cardiac fibroblasts activation by up‐regulating cyclin D1 and stabilizing β‐catenin in vitro
title_full Ubiquitin‐specific protease 2 regulates Ang Ⅱ–induced cardiac fibroblasts activation by up‐regulating cyclin D1 and stabilizing β‐catenin in vitro
title_fullStr Ubiquitin‐specific protease 2 regulates Ang Ⅱ–induced cardiac fibroblasts activation by up‐regulating cyclin D1 and stabilizing β‐catenin in vitro
title_full_unstemmed Ubiquitin‐specific protease 2 regulates Ang Ⅱ–induced cardiac fibroblasts activation by up‐regulating cyclin D1 and stabilizing β‐catenin in vitro
title_short Ubiquitin‐specific protease 2 regulates Ang Ⅱ–induced cardiac fibroblasts activation by up‐regulating cyclin D1 and stabilizing β‐catenin in vitro
title_sort ubiquitin‐specific protease 2 regulates ang ⅱ–induced cardiac fibroblasts activation by up‐regulating cyclin d1 and stabilizing β‐catenin in vitro
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812274/
https://www.ncbi.nlm.nih.gov/pubmed/33314748
http://dx.doi.org/10.1111/jcmm.16162
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