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Effective ribosomal RNA depletion for single-cell total RNA-seq by scDASH

A decade since its invention, single-cell RNA sequencing (scRNA-seq) has become a mainstay technology for profiling transcriptional heterogeneity in individual cells. Yet, most existing scRNA-seq methods capture only polyadenylated mRNA to avoid the cost of sequencing non-messenger transcripts, such...

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Detalles Bibliográficos
Autores principales: Loi, Danson S.C., Yu, Lei, Wu, Angela R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812930/
https://www.ncbi.nlm.nih.gov/pubmed/33520469
http://dx.doi.org/10.7717/peerj.10717