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Donor KIR3DL1/receptor HLA-Bw4-80I Combination Reduces Acute Leukemia Relapse after Umbilical Cord Blood Transplantation without in Vitro T-cell Depletion

BACKGROUND: Donor natural killer (NK) cell alloreactivity in umbilical cord bone marrow transplantation (UCBT) can lead to leukemic relapse. However, NK cell function is calibrated by interaction with human leukocyte antigens (HLAs). This study aimed to investigate graft-resistant leukemia after tra...

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Autores principales: Fang, Xinchen, Zhu, Xiaoyu, Tang, Baolin, Song, Kaidi, Yao, Wen, Wan, Xiang, Liu, Huilan, Peng, Jun, Sun, Zimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Università Cattolica del Sacro Cuore 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813285/
https://www.ncbi.nlm.nih.gov/pubmed/33489044
http://dx.doi.org/10.4084/MJHID.2021.005
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author Fang, Xinchen
Zhu, Xiaoyu
Tang, Baolin
Song, Kaidi
Yao, Wen
Wan, Xiang
Liu, Huilan
Peng, Jun
Sun, Zimin
author_facet Fang, Xinchen
Zhu, Xiaoyu
Tang, Baolin
Song, Kaidi
Yao, Wen
Wan, Xiang
Liu, Huilan
Peng, Jun
Sun, Zimin
author_sort Fang, Xinchen
collection PubMed
description BACKGROUND: Donor natural killer (NK) cell alloreactivity in umbilical cord bone marrow transplantation (UCBT) can lead to leukemic relapse. However, NK cell function is calibrated by interaction with human leukocyte antigens (HLAs). This study aimed to investigate graft-resistant leukemia after transplantation and compared specific genotypes of killer immunoglobulin-like receptors (KIRs) in donors and human leukocyte antigen ligands in patients. METHODS: We retrospectively analyzed 232 patients with acute leukemia from a single center. Patients had undergone UCBT with myeloablative conditioning and without anti-thymocyte globulin. We identified the KIR genotypes of cord blood donors using polymerase chain reaction with sequence-specific primers. All of the donors contained KIR3DL1. RESULTS: The patients were divided into three groups according to the HLA-B locus. The donor KIR3DL1 and recipient HLA-Bw4-80I combination was predictive of being highly educated and was associated with a lower relapse (P=0.006) and better overall survival (probability of relapse=0.13, P < 0.001) than the uneducated group. We found no significant increase in the incidence of acute or chronic graft-versus-host disease. CONCLUSIONS: Our data suggest that the donor KIR3DL1/receptor and HLA-Bw4-80I combination in UCBT results in stronger graft-versus-leukemia effects and improved outcomes in patients with acute leukemia.
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spelling pubmed-78132852021-01-22 Donor KIR3DL1/receptor HLA-Bw4-80I Combination Reduces Acute Leukemia Relapse after Umbilical Cord Blood Transplantation without in Vitro T-cell Depletion Fang, Xinchen Zhu, Xiaoyu Tang, Baolin Song, Kaidi Yao, Wen Wan, Xiang Liu, Huilan Peng, Jun Sun, Zimin Mediterr J Hematol Infect Dis Original Article BACKGROUND: Donor natural killer (NK) cell alloreactivity in umbilical cord bone marrow transplantation (UCBT) can lead to leukemic relapse. However, NK cell function is calibrated by interaction with human leukocyte antigens (HLAs). This study aimed to investigate graft-resistant leukemia after transplantation and compared specific genotypes of killer immunoglobulin-like receptors (KIRs) in donors and human leukocyte antigen ligands in patients. METHODS: We retrospectively analyzed 232 patients with acute leukemia from a single center. Patients had undergone UCBT with myeloablative conditioning and without anti-thymocyte globulin. We identified the KIR genotypes of cord blood donors using polymerase chain reaction with sequence-specific primers. All of the donors contained KIR3DL1. RESULTS: The patients were divided into three groups according to the HLA-B locus. The donor KIR3DL1 and recipient HLA-Bw4-80I combination was predictive of being highly educated and was associated with a lower relapse (P=0.006) and better overall survival (probability of relapse=0.13, P < 0.001) than the uneducated group. We found no significant increase in the incidence of acute or chronic graft-versus-host disease. CONCLUSIONS: Our data suggest that the donor KIR3DL1/receptor and HLA-Bw4-80I combination in UCBT results in stronger graft-versus-leukemia effects and improved outcomes in patients with acute leukemia. Università Cattolica del Sacro Cuore 2021-01-01 /pmc/articles/PMC7813285/ /pubmed/33489044 http://dx.doi.org/10.4084/MJHID.2021.005 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by-nc/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Fang, Xinchen
Zhu, Xiaoyu
Tang, Baolin
Song, Kaidi
Yao, Wen
Wan, Xiang
Liu, Huilan
Peng, Jun
Sun, Zimin
Donor KIR3DL1/receptor HLA-Bw4-80I Combination Reduces Acute Leukemia Relapse after Umbilical Cord Blood Transplantation without in Vitro T-cell Depletion
title Donor KIR3DL1/receptor HLA-Bw4-80I Combination Reduces Acute Leukemia Relapse after Umbilical Cord Blood Transplantation without in Vitro T-cell Depletion
title_full Donor KIR3DL1/receptor HLA-Bw4-80I Combination Reduces Acute Leukemia Relapse after Umbilical Cord Blood Transplantation without in Vitro T-cell Depletion
title_fullStr Donor KIR3DL1/receptor HLA-Bw4-80I Combination Reduces Acute Leukemia Relapse after Umbilical Cord Blood Transplantation without in Vitro T-cell Depletion
title_full_unstemmed Donor KIR3DL1/receptor HLA-Bw4-80I Combination Reduces Acute Leukemia Relapse after Umbilical Cord Blood Transplantation without in Vitro T-cell Depletion
title_short Donor KIR3DL1/receptor HLA-Bw4-80I Combination Reduces Acute Leukemia Relapse after Umbilical Cord Blood Transplantation without in Vitro T-cell Depletion
title_sort donor kir3dl1/receptor hla-bw4-80i combination reduces acute leukemia relapse after umbilical cord blood transplantation without in vitro t-cell depletion
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813285/
https://www.ncbi.nlm.nih.gov/pubmed/33489044
http://dx.doi.org/10.4084/MJHID.2021.005
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