Washing with alkaline solutions in protein A purification improves physicochemical properties of monoclonal antibodies

Protein A affinity chromatography has been widely used for both laboratory scale purification and commercial manufacturing of monoclonal antibodies and Fc-fusion proteins. Protein A purification is specific and efficient. However, there still remain several issues to be addressed, such as incomplete...

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Detalles Bibliográficos
Autores principales: Imura, Yuichi, Tagawa, Toshiaki, Miyamoto, Yuya, Nonoyama, Satoshi, Sumichika, Hiroshi, Fujino, Yasuhiro, Yamanouchi, Masaya, Miki, Hideo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815873/
https://www.ncbi.nlm.nih.gov/pubmed/33469121
http://dx.doi.org/10.1038/s41598-021-81366-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815873/
https://www.ncbi.nlm.nih.gov/pubmed/33469121
http://dx.doi.org/10.1038/s41598-021-81366-6

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