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CRISPRcruncher: A tool for engineering restriction sites into coding regions

CRISPR/Cas9 genome editing strategies often rely on the placement of an introduced restriction endonuclease (RE) site adjacent to the genomic edit of interest. This allows for rapid initial PCR-based detection of cells and organisms containing the edit of interest and may also be used for subsequent...

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Detalles Bibliográficos
Autores principales: Fay, Samuel F., Fay, David S., Chhatre, Vikram E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816087/
https://www.ncbi.nlm.nih.gov/pubmed/33490886
http://dx.doi.org/10.17912/micropub.biology.000343