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CRISPRcruncher: A tool for engineering restriction sites into coding regions
CRISPR/Cas9 genome editing strategies often rely on the placement of an introduced restriction endonuclease (RE) site adjacent to the genomic edit of interest. This allows for rapid initial PCR-based detection of cells and organisms containing the edit of interest and may also be used for subsequent...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Caltech Library
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816087/ https://www.ncbi.nlm.nih.gov/pubmed/33490886 http://dx.doi.org/10.17912/micropub.biology.000343 |