Delivering defined amounts of purified protein with high precision into living cells

Here, we detail a protocol using electroporation to precisely deliver defined amounts of purified protein into CAD cells. This method allows one million cells to be electroporated with protein simultaneously, with high delivery efficiency and low cell death. Further, by circumventing the normal bios...

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Detalles Bibliográficos
Autores principales: Skruber, Kristen, Read, Tracy-Ann, Vitriol, Eric A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817494/
https://www.ncbi.nlm.nih.gov/pubmed/33511357
http://dx.doi.org/10.1016/j.xpro.2020.100272

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817494/
https://www.ncbi.nlm.nih.gov/pubmed/33511357
http://dx.doi.org/10.1016/j.xpro.2020.100272

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