Cargando…
Purification of Recombinant α-synuclein: A Comparison of Commonly Used Protocols
[Image: see text] The initial state of the intrinsically disordered protein α-synuclein (aSyn), e.g., the presence of oligomers and degradation products, or the presence of contaminants and adducts can greatly influence the aggregation kinetics and toxicity of the protein. Here, we compare four comm...
Autores principales: | Stephens, Amberley D., Matak-Vinkovic, Dijana, Fernandez-Villegas, Ana, Kaminski Schierle, Gabriele S. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical Society
2020
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818547/ https://www.ncbi.nlm.nih.gov/pubmed/33237763 http://dx.doi.org/10.1021/acs.biochem.0c00725 |
Ejemplares similares
-
Fast Purification of Recombinant Monomeric Amyloid-β
from E. coli and Amyloid-β-mCherry Aggregates
from Mammalian Cells
por: Stephens, Amberley D., et al.
Publicado: (2020) -
Different Structural Conformers of Monomeric α-Synuclein
Identified after Lyophilizing and Freezing
por: Stephens, Amberley D., et al.
Publicado: (2018) -
Millisecond
Hydrogen/Deuterium-Exchange Mass Spectrometry
Approach to Correlate Local Structure and Aggregation in α-Synuclein
por: Seetaloo, Neeleema, et al.
Publicado: (2022) -
Intramitochondrial proteostasis is directly coupled to α-synuclein and amyloid β1-42 pathologies
por: Lautenschläger, Janin, et al.
Publicado: (2020) -
Structural basis of synaptic vesicle assembly promoted by α-synuclein
por: Fusco, Giuliana, et al.
Publicado: (2016)