Multiple freeze-thaw cycles lead to a loss of consistency in poly(A)-enriched RNA sequencing

BACKGROUND: Both RNA-Seq and sample freeze-thaw are ubiquitous. However, knowledge about the impact of freeze-thaw on downstream analyses is limited. The lack of common quality metrics that are sufficiently sensitive to freeze-thaw and RNA degradation, e.g. the RNA Integrity Score, makes such assess...

Descripción completa

Detalles Bibliográficos
Autores principales: Kellman, Benjamin P., Baghdassarian, Hratch M., Pramparo, Tiziano, Shamie, Isaac, Gazestani, Vahid, Begzati, Arjana, Li, Shangzhong, Nalabolu, Srinivasa, Murray, Sarah, Lopez, Linda, Pierce, Karen, Courchesne, Eric, Lewis, Nathan E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818915/
https://www.ncbi.nlm.nih.gov/pubmed/33478392
http://dx.doi.org/10.1186/s12864-021-07381-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818915/
https://www.ncbi.nlm.nih.gov/pubmed/33478392
http://dx.doi.org/10.1186/s12864-021-07381-z

Ejemplares similares