Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying functional circRNAs

Short hairpin RNAs (shRNAs) are used to deplete circRNAs by targeting back-splicing junction (BSJ) sites. However, frequent discrepancies exist between shRNA-mediated circRNA knockdown and the corresponding biological effect, querying their robustness. By leveraging CRISPR/Cas13d tool and optimizing...

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Detalles Bibliográficos
Autores principales: Zhang, Yang, Nguyen, Tuan M., Zhang, Xiao-Ou, Wang, Limei, Phan, Tin, Clohessy, John G., Pandolfi, Pier Paolo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818937/
https://www.ncbi.nlm.nih.gov/pubmed/33478577
http://dx.doi.org/10.1186/s13059-021-02263-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818937/
https://www.ncbi.nlm.nih.gov/pubmed/33478577
http://dx.doi.org/10.1186/s13059-021-02263-9

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