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In vitro synthesis of 32 translation-factor proteins from a single template reveals impaired ribosomal processivity

The Protein synthesis Using Recombinant Elements (PURE) system enables transcription and translation of a DNA template from purified components. Therefore, the PURE system-catalyzed generation of RNAs and proteins constituting the PURE system itself represents a major challenge toward a self-replica...

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Detalles Bibliográficos
Autores principales: Doerr, Anne, Foschepoth, David, Forster, Anthony C., Danelon, Christophe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820420/
https://www.ncbi.nlm.nih.gov/pubmed/33479285
http://dx.doi.org/10.1038/s41598-020-80827-8