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In vitro synthesis of 32 translation-factor proteins from a single template reveals impaired ribosomal processivity
The Protein synthesis Using Recombinant Elements (PURE) system enables transcription and translation of a DNA template from purified components. Therefore, the PURE system-catalyzed generation of RNAs and proteins constituting the PURE system itself represents a major challenge toward a self-replica...
Autores principales: | Doerr, Anne, Foschepoth, David, Forster, Anthony C., Danelon, Christophe |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820420/ https://www.ncbi.nlm.nih.gov/pubmed/33479285 http://dx.doi.org/10.1038/s41598-020-80827-8 |
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