Effect of mutation at oxyanion hole residu (H110F) on activity of Lk4 lipase

Mutant of lipase at oxyanion hole (H110 F) was constructed. The gene was highly expressed in Eschericia coli BL21 (DE3) and the recombinant protein was purified using Ni-NTA affinity chromatography. The activity of mutant enzyme was significantly increased compared to that the wild type. Further com...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma’ruf, Ilma Fauziah, Widhiastuty, Made Puspasari, Suharti, Moeis, Maelita Ramdani, Akhmaloka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823203/
https://www.ncbi.nlm.nih.gov/pubmed/33532247
http://dx.doi.org/10.1016/j.btre.2021.e00590

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823203/
https://www.ncbi.nlm.nih.gov/pubmed/33532247
http://dx.doi.org/10.1016/j.btre.2021.e00590

Ejemplares similares