Cargando…
Effect of mutation at oxyanion hole residu (H110F) on activity of Lk4 lipase
Mutant of lipase at oxyanion hole (H110 F) was constructed. The gene was highly expressed in Eschericia coli BL21 (DE3) and the recombinant protein was purified using Ni-NTA affinity chromatography. The activity of mutant enzyme was significantly increased compared to that the wild type. Further com...
| Autores principales: | , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823203/ https://www.ncbi.nlm.nih.gov/pubmed/33532247 http://dx.doi.org/10.1016/j.btre.2021.e00590 |