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Efficient Cryopreservation of Populus tremula by In Vitro-Grown Axillary Buds and Genetic Stability of Recovered Plants

Axillary buds of in vitro microshoots were successfully frozen at –196 °C by the one-step freezing method using the protective vitrification solution 2 (PVS2). Microshoots were taken from 11 transgenic lines and three wild type lines. Influence of different explant pretreatments were analyzed from t...

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Detalles Bibliográficos
Autores principales: Vidyagina, Elena O., Kharchenko, Nikolay N., Shestibratov, Konstantin A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823548/
https://www.ncbi.nlm.nih.gov/pubmed/33401697
http://dx.doi.org/10.3390/plants10010077