Effects of Maternal Fiber Intake on Intestinal Morphology, Bacterial Profile and Proteome of Newborns Using Pig as Model

Dietary fiber intake during pregnancy may improve offspring intestinal development. The aim of this study was to evaluate the effect of maternal high fiber intake during late gestation on intestinal morphology, microbiota, and intestinal proteome of newborn piglets. Sixteen sows were randomly alloca...

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Autores principales: He, Ying, Peng, Xie, Liu, Yang, Wu, Qing, Zhou, Qiang, Hu, Liang, Fang, Zhengfeng, Lin, Yan, Xu, Shengyu, Feng, Bin, Li, Jian, Zhuo, Yong, Wu, De, Che, Lianqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823571/
https://www.ncbi.nlm.nih.gov/pubmed/33375592
http://dx.doi.org/10.3390/nu13010042
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author He, Ying
Peng, Xie
Liu, Yang
Wu, Qing
Zhou, Qiang
Hu, Liang
Fang, Zhengfeng
Lin, Yan
Xu, Shengyu
Feng, Bin
Li, Jian
Zhuo, Yong
Wu, De
Che, Lianqiang
author_facet He, Ying
Peng, Xie
Liu, Yang
Wu, Qing
Zhou, Qiang
Hu, Liang
Fang, Zhengfeng
Lin, Yan
Xu, Shengyu
Feng, Bin
Li, Jian
Zhuo, Yong
Wu, De
Che, Lianqiang
author_sort He, Ying
collection PubMed
description Dietary fiber intake during pregnancy may improve offspring intestinal development. The aim of this study was to evaluate the effect of maternal high fiber intake during late gestation on intestinal morphology, microbiota, and intestinal proteome of newborn piglets. Sixteen sows were randomly allocated into two groups receiving the control diet (CD) and high-fiber diet (HFD) from day 90 of gestation to farrowing. Newborn piglets were selected from each litter, named as CON and Fiber group, respectively. Maternal high fiber intake did not markedly improve the birth weight, but increased the body length, the ileal crypt depth and colonic acetate level. In addition, maternal high fiber intake increased the α-diversity indices (Observed species, Simpson, and ACE), and the abundance of Acidobacteria and Bacteroidetes at phylum level, significantly increased the abundance of Bradyrhizobium and Phyllobacterium at genus level in the colon of newborn piglets. Moreover, maternal high fiber intake markedly altered the ileal proteome, increasing the abundances of proteins associated with oxidative status, energy metabolism, and immune and inflammatory responses, and decreasing abundances of proteins related to cellular apoptosis, cell structure, and motility. These findings indicated that maternal high fiber intake could alter intestinal morphology, along with the altered intestinal microbiota composition and proteome of offspring.
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spelling pubmed-78235712021-01-24 Effects of Maternal Fiber Intake on Intestinal Morphology, Bacterial Profile and Proteome of Newborns Using Pig as Model He, Ying Peng, Xie Liu, Yang Wu, Qing Zhou, Qiang Hu, Liang Fang, Zhengfeng Lin, Yan Xu, Shengyu Feng, Bin Li, Jian Zhuo, Yong Wu, De Che, Lianqiang Nutrients Article Dietary fiber intake during pregnancy may improve offspring intestinal development. The aim of this study was to evaluate the effect of maternal high fiber intake during late gestation on intestinal morphology, microbiota, and intestinal proteome of newborn piglets. Sixteen sows were randomly allocated into two groups receiving the control diet (CD) and high-fiber diet (HFD) from day 90 of gestation to farrowing. Newborn piglets were selected from each litter, named as CON and Fiber group, respectively. Maternal high fiber intake did not markedly improve the birth weight, but increased the body length, the ileal crypt depth and colonic acetate level. In addition, maternal high fiber intake increased the α-diversity indices (Observed species, Simpson, and ACE), and the abundance of Acidobacteria and Bacteroidetes at phylum level, significantly increased the abundance of Bradyrhizobium and Phyllobacterium at genus level in the colon of newborn piglets. Moreover, maternal high fiber intake markedly altered the ileal proteome, increasing the abundances of proteins associated with oxidative status, energy metabolism, and immune and inflammatory responses, and decreasing abundances of proteins related to cellular apoptosis, cell structure, and motility. These findings indicated that maternal high fiber intake could alter intestinal morphology, along with the altered intestinal microbiota composition and proteome of offspring. MDPI 2020-12-25 /pmc/articles/PMC7823571/ /pubmed/33375592 http://dx.doi.org/10.3390/nu13010042 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
He, Ying
Peng, Xie
Liu, Yang
Wu, Qing
Zhou, Qiang
Hu, Liang
Fang, Zhengfeng
Lin, Yan
Xu, Shengyu
Feng, Bin
Li, Jian
Zhuo, Yong
Wu, De
Che, Lianqiang
Effects of Maternal Fiber Intake on Intestinal Morphology, Bacterial Profile and Proteome of Newborns Using Pig as Model
title Effects of Maternal Fiber Intake on Intestinal Morphology, Bacterial Profile and Proteome of Newborns Using Pig as Model
title_full Effects of Maternal Fiber Intake on Intestinal Morphology, Bacterial Profile and Proteome of Newborns Using Pig as Model
title_fullStr Effects of Maternal Fiber Intake on Intestinal Morphology, Bacterial Profile and Proteome of Newborns Using Pig as Model
title_full_unstemmed Effects of Maternal Fiber Intake on Intestinal Morphology, Bacterial Profile and Proteome of Newborns Using Pig as Model
title_short Effects of Maternal Fiber Intake on Intestinal Morphology, Bacterial Profile and Proteome of Newborns Using Pig as Model
title_sort effects of maternal fiber intake on intestinal morphology, bacterial profile and proteome of newborns using pig as model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823571/
https://www.ncbi.nlm.nih.gov/pubmed/33375592
http://dx.doi.org/10.3390/nu13010042
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