Customized optical mapping by CRISPR–Cas9 mediated DNA labeling with multiple sgRNAs

Whole-genome mapping technologies have been developed as a complementary tool to provide scaffolds for genome assembly and structural variation analysis (1,2). We recently introduced a novel DNA labeling strategy based on a CRISPR–Cas9 genome editing system, which can target any 20bp sequences. The...

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Detalles Bibliográficos
Autores principales: Abid, Heba Z, Young, Eleanor, McCaffrey, Jennifer, Raseley, Kaitlin, Varapula, Dharma, Wang, Hung-Yi, Piazza, Danielle, Mell, Joshua, Xiao, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826249/
https://www.ncbi.nlm.nih.gov/pubmed/33231685
http://dx.doi.org/10.1093/nar/gkaa1088

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826249/
https://www.ncbi.nlm.nih.gov/pubmed/33231685
http://dx.doi.org/10.1093/nar/gkaa1088

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