A fast and robust iterative genome-editing method based on a Rock-Paper-Scissors strategy

The production of optimized strains of a specific phenotype requires the construction and testing of a large number of genome modifications and combinations thereof. Most bacterial iterative genome-editing methods include essential steps to eliminate selection markers, or to cure plasmids. Additiona...

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Detalles Bibliográficos
Autores principales: Wang, Jichao, Sui, Xinyue, Ding, Yamei, Fu, Yingxin, Feng, Xinjun, Liu, Min, Zhang, Youming, Xian, Mo, Zhao, Guang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826264/
https://www.ncbi.nlm.nih.gov/pubmed/33270888
http://dx.doi.org/10.1093/nar/gkaa1141

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826264/
https://www.ncbi.nlm.nih.gov/pubmed/33270888
http://dx.doi.org/10.1093/nar/gkaa1141

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