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A fast and robust iterative genome-editing method based on a Rock-Paper-Scissors strategy
The production of optimized strains of a specific phenotype requires the construction and testing of a large number of genome modifications and combinations thereof. Most bacterial iterative genome-editing methods include essential steps to eliminate selection markers, or to cure plasmids. Additiona...
| Autores principales: | , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826264/ https://www.ncbi.nlm.nih.gov/pubmed/33270888 http://dx.doi.org/10.1093/nar/gkaa1141 |