Catalytically inactive Cas9 impairs DNA replication fork progression to induce focal genomic instability

Catalytically inactive Cas9 (dCas9) has become an increasingly popular tool for targeted gene activation/inactivation, live-cell imaging, and base editing. While dCas9 was reported to induce base substitutions and indels, it has not been associated with structural variations. Here, we show that dCas...

Descripción completa

Detalles Bibliográficos
Autores principales: Doi, Goro, Okada, Satoshi, Yasukawa, Takehiro, Sugiyama, Yuki, Bala, Siqin, Miyazaki, Shintaro, Kang, Dongchon, Ito, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Genome Integrity, Repair and Replication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826275/
https://www.ncbi.nlm.nih.gov/pubmed/33398345
http://dx.doi.org/10.1093/nar/gkaa1241

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826275/
https://www.ncbi.nlm.nih.gov/pubmed/33398345
http://dx.doi.org/10.1093/nar/gkaa1241

Ejemplares similares