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Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for Lactobacillus brevis

Lactobacillus brevis is the most common bacteria that causes beer spoilage. In this work, a novel electrochemical immunosensor was fabricated for ultra-sensitive determination of L. brevis. Gold nanoparticles (AuNPs) were firstly electro-deposited on the electrode surface for enhancing the electro-c...

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Detalles Bibliográficos
Autor principal: Zhao, Le
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827081/
https://www.ncbi.nlm.nih.gov/pubmed/33445448
http://dx.doi.org/10.3390/mi12010075
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author Zhao, Le
author_facet Zhao, Le
author_sort Zhao, Le
collection PubMed
description Lactobacillus brevis is the most common bacteria that causes beer spoilage. In this work, a novel electrochemical immunosensor was fabricated for ultra-sensitive determination of L. brevis. Gold nanoparticles (AuNPs) were firstly electro-deposited on the electrode surface for enhancing the electro-conductivity and specific surface area. Ionic liquid was used for improving the immobilization performance of the immunosensor. After optimization, a linear regression equation can be observed between the ∆(current) and concentration of L. brevis from 10(4) CFU/mL to 10(9) CFU/mL. The limit of detection can be estimated to be 10(3) CFU/mL.
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spelling pubmed-78270812021-01-25 Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for Lactobacillus brevis Zhao, Le Micromachines (Basel) Article Lactobacillus brevis is the most common bacteria that causes beer spoilage. In this work, a novel electrochemical immunosensor was fabricated for ultra-sensitive determination of L. brevis. Gold nanoparticles (AuNPs) were firstly electro-deposited on the electrode surface for enhancing the electro-conductivity and specific surface area. Ionic liquid was used for improving the immobilization performance of the immunosensor. After optimization, a linear regression equation can be observed between the ∆(current) and concentration of L. brevis from 10(4) CFU/mL to 10(9) CFU/mL. The limit of detection can be estimated to be 10(3) CFU/mL. MDPI 2021-01-12 /pmc/articles/PMC7827081/ /pubmed/33445448 http://dx.doi.org/10.3390/mi12010075 Text en © 2021 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Le
Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for Lactobacillus brevis
title Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for Lactobacillus brevis
title_full Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for Lactobacillus brevis
title_fullStr Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for Lactobacillus brevis
title_full_unstemmed Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for Lactobacillus brevis
title_short Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for Lactobacillus brevis
title_sort horseradish peroxidase labelled-sandwich electrochemical sensor based on ionic liquid-gold nanoparticles for lactobacillus brevis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827081/
https://www.ncbi.nlm.nih.gov/pubmed/33445448
http://dx.doi.org/10.3390/mi12010075
work_keys_str_mv AT zhaole horseradishperoxidaselabelledsandwichelectrochemicalsensorbasedonionicliquidgoldnanoparticlesforlactobacillusbrevis