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Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for Lactobacillus brevis
Lactobacillus brevis is the most common bacteria that causes beer spoilage. In this work, a novel electrochemical immunosensor was fabricated for ultra-sensitive determination of L. brevis. Gold nanoparticles (AuNPs) were firstly electro-deposited on the electrode surface for enhancing the electro-c...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827081/ https://www.ncbi.nlm.nih.gov/pubmed/33445448 http://dx.doi.org/10.3390/mi12010075 |
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author | Zhao, Le |
author_facet | Zhao, Le |
author_sort | Zhao, Le |
collection | PubMed |
description | Lactobacillus brevis is the most common bacteria that causes beer spoilage. In this work, a novel electrochemical immunosensor was fabricated for ultra-sensitive determination of L. brevis. Gold nanoparticles (AuNPs) were firstly electro-deposited on the electrode surface for enhancing the electro-conductivity and specific surface area. Ionic liquid was used for improving the immobilization performance of the immunosensor. After optimization, a linear regression equation can be observed between the ∆(current) and concentration of L. brevis from 10(4) CFU/mL to 10(9) CFU/mL. The limit of detection can be estimated to be 10(3) CFU/mL. |
format | Online Article Text |
id | pubmed-7827081 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78270812021-01-25 Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for Lactobacillus brevis Zhao, Le Micromachines (Basel) Article Lactobacillus brevis is the most common bacteria that causes beer spoilage. In this work, a novel electrochemical immunosensor was fabricated for ultra-sensitive determination of L. brevis. Gold nanoparticles (AuNPs) were firstly electro-deposited on the electrode surface for enhancing the electro-conductivity and specific surface area. Ionic liquid was used for improving the immobilization performance of the immunosensor. After optimization, a linear regression equation can be observed between the ∆(current) and concentration of L. brevis from 10(4) CFU/mL to 10(9) CFU/mL. The limit of detection can be estimated to be 10(3) CFU/mL. MDPI 2021-01-12 /pmc/articles/PMC7827081/ /pubmed/33445448 http://dx.doi.org/10.3390/mi12010075 Text en © 2021 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhao, Le Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for Lactobacillus brevis |
title | Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for Lactobacillus brevis |
title_full | Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for Lactobacillus brevis |
title_fullStr | Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for Lactobacillus brevis |
title_full_unstemmed | Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for Lactobacillus brevis |
title_short | Horseradish Peroxidase Labelled-Sandwich Electrochemical Sensor Based on Ionic Liquid-Gold Nanoparticles for Lactobacillus brevis |
title_sort | horseradish peroxidase labelled-sandwich electrochemical sensor based on ionic liquid-gold nanoparticles for lactobacillus brevis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827081/ https://www.ncbi.nlm.nih.gov/pubmed/33445448 http://dx.doi.org/10.3390/mi12010075 |
work_keys_str_mv | AT zhaole horseradishperoxidaselabelledsandwichelectrochemicalsensorbasedonionicliquidgoldnanoparticlesforlactobacillusbrevis |