Redox Status in Canine Leishmaniasis

SIMPLE SUMMARY: Leishmaniasis is under strict observation by World Health Organization but its pathogenesis has not been completely clarified yet. Our aim was to compare healthy and affected dogs measuring parameters related to oxidative stress, namely reactive oxygen species, reactive nitrogen spec...

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Autores principales: Quintavalla, Fausto, Basini, Giuseppina, Bussolati, Simona, Carrozzo, Gennaro Giuseppe, Inglese, Antonio, Ramoni, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7828002/
https://www.ncbi.nlm.nih.gov/pubmed/33429894
http://dx.doi.org/10.3390/ani11010119
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author Quintavalla, Fausto
Basini, Giuseppina
Bussolati, Simona
Carrozzo, Gennaro Giuseppe
Inglese, Antonio
Ramoni, Roberto
author_facet Quintavalla, Fausto
Basini, Giuseppina
Bussolati, Simona
Carrozzo, Gennaro Giuseppe
Inglese, Antonio
Ramoni, Roberto
author_sort Quintavalla, Fausto
collection PubMed
description SIMPLE SUMMARY: Leishmaniasis is under strict observation by World Health Organization but its pathogenesis has not been completely clarified yet. Our aim was to compare healthy and affected dogs measuring parameters related to oxidative stress, namely reactive oxygen species, reactive nitrogen species and scavenger activities, using colorimetric assays. Our results demonstrate that several of the examined parameters are modified in canine Leishmaniasis. Therefore, it is essential to further investigate this topic to shed light on the pathogenesis of the disease. ABSTRACT: The World Health Organization defined leishmaniasis as one of the priority attention diseases. Aiming to clarify some aspects of its pathogenetic mechanisms, our study focused on the assessment of redox status in dogs, the main reservoir for Leishmania infantum. Forty-five dogs from an endemic area in southern Italy were divided into four different groups (from mild disease with negative to low positive antibody levels to very severe disease with medium to high positive antibody levels) according to the LeishVet group guidelines. Their plasma and/or sera were tested for reactive oxygen species (ROS), namely the superoxide anion (O(2)(−)), reactive nitrogen species (RNS), such as nitric oxide (NO) and hydroperoxides (ROOH), as well as activity of the detoxifying enzyme superoxide dismutase (SOD), and total nonenzymatic antioxidant capacity, as determined by the ferric reducing-antioxidant power (FRAP) assay. O(2)(−) generation was significantly (p < 0.05) reduced in leishmaniasis-affected dogs independently of the clinical stage, while NO production was stimulated (p < 0.05) only in II and III stage patients. No difference could be found for the levels of hydroperoxides and SOD activity between healthy and pathological subjects. FRAP values were lower in affected dogs but only in stage II. Taken together, although we demonstrated that several redox status parameters are altered in the plasma of dog affected by leishmaniasis, the oxidative stress changes that are observed in this disease, are possibly mainly due to cellular blood components i.e., neutrophils responsible for the elimination of the parasite. Further studies are required to assess the clinical values of the collected data.
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spelling pubmed-78280022021-01-25 Redox Status in Canine Leishmaniasis Quintavalla, Fausto Basini, Giuseppina Bussolati, Simona Carrozzo, Gennaro Giuseppe Inglese, Antonio Ramoni, Roberto Animals (Basel) Article SIMPLE SUMMARY: Leishmaniasis is under strict observation by World Health Organization but its pathogenesis has not been completely clarified yet. Our aim was to compare healthy and affected dogs measuring parameters related to oxidative stress, namely reactive oxygen species, reactive nitrogen species and scavenger activities, using colorimetric assays. Our results demonstrate that several of the examined parameters are modified in canine Leishmaniasis. Therefore, it is essential to further investigate this topic to shed light on the pathogenesis of the disease. ABSTRACT: The World Health Organization defined leishmaniasis as one of the priority attention diseases. Aiming to clarify some aspects of its pathogenetic mechanisms, our study focused on the assessment of redox status in dogs, the main reservoir for Leishmania infantum. Forty-five dogs from an endemic area in southern Italy were divided into four different groups (from mild disease with negative to low positive antibody levels to very severe disease with medium to high positive antibody levels) according to the LeishVet group guidelines. Their plasma and/or sera were tested for reactive oxygen species (ROS), namely the superoxide anion (O(2)(−)), reactive nitrogen species (RNS), such as nitric oxide (NO) and hydroperoxides (ROOH), as well as activity of the detoxifying enzyme superoxide dismutase (SOD), and total nonenzymatic antioxidant capacity, as determined by the ferric reducing-antioxidant power (FRAP) assay. O(2)(−) generation was significantly (p < 0.05) reduced in leishmaniasis-affected dogs independently of the clinical stage, while NO production was stimulated (p < 0.05) only in II and III stage patients. No difference could be found for the levels of hydroperoxides and SOD activity between healthy and pathological subjects. FRAP values were lower in affected dogs but only in stage II. Taken together, although we demonstrated that several redox status parameters are altered in the plasma of dog affected by leishmaniasis, the oxidative stress changes that are observed in this disease, are possibly mainly due to cellular blood components i.e., neutrophils responsible for the elimination of the parasite. Further studies are required to assess the clinical values of the collected data. MDPI 2021-01-08 /pmc/articles/PMC7828002/ /pubmed/33429894 http://dx.doi.org/10.3390/ani11010119 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Quintavalla, Fausto
Basini, Giuseppina
Bussolati, Simona
Carrozzo, Gennaro Giuseppe
Inglese, Antonio
Ramoni, Roberto
Redox Status in Canine Leishmaniasis
title Redox Status in Canine Leishmaniasis
title_full Redox Status in Canine Leishmaniasis
title_fullStr Redox Status in Canine Leishmaniasis
title_full_unstemmed Redox Status in Canine Leishmaniasis
title_short Redox Status in Canine Leishmaniasis
title_sort redox status in canine leishmaniasis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7828002/
https://www.ncbi.nlm.nih.gov/pubmed/33429894
http://dx.doi.org/10.3390/ani11010119
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